The complete flow cytometry guide
Flow cytometry is a popular laser-based technology to analyze the characteristics of cells or particles. Discover more with our introduction to flow cytometry.
Flow cytometry is a widely used method for analyzing the expression of cell surface and intracellular molecules, characterizing and defining different cell types in a heterogeneous cell population, assessing the purity of isolated subpopulations, and analyzing cell size and volume. It allows simultaneous multi-parameter analysis of single cells.
It is predominantly used to measure fluorescence intensity produced by fluorescent-labeled antibodies detecting proteins, or ligands that bind to specific cell-associated molecules such as propidium iodide binding to DNA.
The staining procedure involves making a single-cell suspension from cell culture or tissue samples. The cells are then incubated in tubes or microtiter plates with unlabeled or fluorochrome-labeled antibodies and analyzed on the flow cytometer.
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In research and clinical settings, flow cytometry is widely used for immunophenotyping, assessing the cellular composition of samples, evaluating cellular function and health, isolating selected cells by sorting, and in biomarker and drug discovery.
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