The complete IHC guide
Easily plan and optimize your IHC experiments with our comprehensive guide.
Discover your comprehensive guide to immunohistochemistry (IHC). From tissue processing and antibody selection to detection, controls, and troubleshooting, this guide will help boost your research.
Essential to a successful IHC experiment is a robust, optimized, and reproducible staining regimen that makes use of high-quality, specific reagents.
Get results fast with validated kits and reagents
For IHC, to ensure that you get the data you need on your first experiment, we recommend our optimized kits and reagents:
- Buffers for heat-mediated and enzymatic antigen retrieval
- Highly sensitive Labeled Streptavidin-Biotin LSAB ABC kits and micro-polymer EXPOSE kits for IHC detection and amplification (for mouse antibodies used on mouse samples, we recommend our Mouse-on-Mouse kit)
- Biotin and micro-polymer conjugated secondary antibodies to construct your own ABC method
- Optimized normal serums for blocking
- IHC validated primary antibodies