The complete IHC guide
Easily plan and optimize your IHC experiments with our comprehensive guide.
Unlock clearer staining, stronger results, and more reproducible data with our comprehensive immunohistochemistry (IHC) guide. Whether you're new to IHC or looking to refine established workflows, this guide brings together expert insights, proven protocols, and practical troubleshooting tips to support every step of your tissue‑based research.
Immunohistochemistry (IHC) is a widely used method for detecting specific proteins within tissue sections using antigen–antibody binding. By combining highly selective antibodies with chromogenic or fluorescent detection systems, IHC allows you to map protein localization, measure expression levels, and explore tissue architecture at cellular resolution.
IHC provides valuable insight into how proteins behave within their native microenvironment, helping you identify biomarkers, validate targets, and understand disease mechanisms with greater precision.
Download the complete IHC guide
Get results fast with validated kits and reagents
For IHC, to ensure that you get the data you need on your first experiment, we recommend our optimized kits and immunohistochemistry reagents:
- Buffers for heat-mediated and enzymatic antigen retrieval
- Highly sensitive Labeled Streptavidin-Biotin LSAB ABC kits and micro-polymer EXPOSE kits for IHC detection and amplification (for mouse antibodies used on mouse samples, we recommend our Mouse-on-Mouse kit)
- Biotin and micro-polymer conjugated secondary antibodies to construct your own ABC method
- Optimized normal serums for blocking
- IHC validated primary antibodies