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The complete IHC guide

Easily plan and optimize your IHC experiments with our comprehensive guide.

Discover your comprehensive guide to immunohistochemistry (IHC). From tissue processing and antibody selection to detection, controls, and troubleshooting, this guide will help boost your research.

Essential to a successful IHC experiment is a robust, optimized, and reproducible staining regimen that makes use of high-quality, specific reagents.

IHC application guide


  • What is IHC?

    Immunohistochemistry is a powerful tool for visualizing the distribution and localization of antigens in tissue sections.

  • Sample preparation

    Sample preparation is key to producing high-quality staining during immunohistochemistry (IHC).

  • Antigen retrieval and permeabilization for IHC

    After sample preparation, two further steps may be required: antigen retrieval and permeabilization.

  • Blocking in IHC

    Blocking is an essential step in your immunohistochemistry (IHC) experiments as it reduces background signals and false positives.

  • Antibodies in IHC

    Choosing the correct antibodies is critical to the success of your IHC experiment.

  • Detection and amplification systems

    In IHC, antigens can be detected by either chromogenic or fluorescent methods.

  • Controls in IHC

    It is essential to run controls in IHC staining experiments to confirm that the observed staining pattern is accurate and reliable.