Once you have differentiated your PSCs into your desired cell type eg neural, cardiac, lung, etc, it is important to check that the cells are no longer expressing the undifferentiated cell markers described above (Oct4, KLF-4, c-Myc, Sox2, etc). You can do this as described in section 2: PSC maintenance using the undifferentiated cell markers described there.

It is also now important to check that the cell types you have generated are the cell types you expect them to be. Here we will list some of the antibodies we recommend that you can use to check the presence of markers on different cell types.

Germ layers

Mesoderm

Brachyury: Recombinant Anti-Brachyury / Bry antibody [EPR18113] (ab209665)

CD31: Recombinant Anti-CD31 antibody [EP3095] (ab134168)

CD34: Recombinant Anti-CD34 antibody [EP373Y] (ab81289)

BMP2: Recombinant Anti-BMP2 antibody [EPR20807] (ab214821)

BMP4: Recombinant Anti-BMP4 antibody [EPR6211] (ab124715)

N-cadherin: Anti-N Cadherin antibody (ab18203)

Nodal: Recombinant Anti-Nodal antibody [EPR2057] (ab109317)

Snail: Recombinant Anti-SNAIL antibody [EPR21043] (ab216347)

Endoderm

Sox17: Recombinant Anti-SOX17 antibody [EPR20684] (ab224637)

FOXA2: Recombinant Anti-FOXA2 antibody [EPR4466] (ab108422)

Ectoderm

Nestin: Recombinant Anti-Nestin antibody [SP103] (ab105389)

Notch1: Recombinant Anti-Notch1 antibody [EP1238Y] (ab52627)

TP63: Recombinant Anti-p63 antibody [EPR5701] (ab124762)

Neural cell types

Neural stem cells

Fas: Recombinant Anti-Fas antibody [EPR5700] (ab133619)

Frizzled-9: Recombinant Anti-Frizzled 9 antibody [EPR4011] (ab108628)

MSI1 (Musashi-1): Recombinant Anti-Musashi 1 / Msi1 antibody [EP1302] (ab52865)

MSl2 (Musashi-2): Recombinant Anti-MSI2 antibody [EP1305Y] (ab76148)

Nestin: Recombinant Anti-Nestin antibody [SP103] (ab105389)

Noggin: Recombinant Anti-Noggin antibody [EPR1561] (ab124977)

Pax-6: Recombinant Anti-PAX6 antibody [EPR15858] (ab195045)

Sox1: Recombinant Anti-SOX1 antibody [EPR4766] (ab109290)

Sox2: Recombinant Anti-SOX2 antibody [SP76] (ab93689)

Neuronal cells

α-Synuclein: Recombinant Anti-Alpha-synuclein antibody [MJFR1] (ab138501)

NCAM: Recombinant Anti-NCAM1 antibody [EP2567Y] (ab75813)

Doublecortin: Recombinant Anti-Doublecortin antibody [EPR10935(B)] (ab167400)

GABA B Receptor: Recombinant Anti-GABA B Receptor 1 antibody [EPR22954-47] (ab238130)

GAP-43: Recombinant Anti-GAP43 antibody [EP890Y] (ab75810)

Glutamine Synthetase: Recombinant Anti-Glutamine Synthetase antibody [EPR13022(B)] (ab176562)

MASH1: Recombinant Anti-MASH1/Achaete-scute homolog 1 antibody [EPR19840] (ab211327)

mGluR1α: Recombinant Anti-mGluR1a antibody [EPR13540] (ab183712)

Nestin: Recombinant Anti-Nestin antibody [SP103] (ab105389)

Nicastrin: Recombinant Anti-Nicastrin antibody [EPR2575Y] (ab68145)

Pax-5: Recombinant Anti-PAX5 antibody [EPR3730(2)] (ab109443)

P-glycoprotein: Recombinant Anti-P Glycoprotein antibody [EPR10364-57] - BSA and Azide free (ab216656)

Synapsin I: Recombinant Anti-Synapsin I (phospho S553) antibody [E377] (ab32532)

Synaptophysin: Recombinant Anti-Synaptophysin antibody [YE269] (ab32127)

Astrocytes

GFAP: Recombinant Anti-GFAP antibody [EPR1034Y] (ab68428)

EAAT1: Recombinant Anti-EAAT1 antibody [EPR12686] (ab181036)

EAAT2: Recombinant Anti-EAAT2 antibody [EPR19794] (ab205247)

Glutamine synthetase: Recombinant Anti-Glutamine Synthetase antibody [EPR13022(B)] (ab176562)

S100 beta: Recombinant Anti-S100 beta antibody [EP1576Y] (ab52642)

ALDH1L1: Recombinant Anti-ALDH1L1 antibody [EPR12743(B)] (ab177463)

Oligodendrocytes

NG2: Recombinant Anti-NG2 antibody [EPR9195] (ab139406)

Olig1: Anti-Olig1 antibody [EPR6790] (ab124908)

Oligodendrocyte specific protein (OSP): Recombinant Anti-Oligodendrocyte Specific Protein antibody [EPR12726] (ab175236)

Myelin basic protein (MBP): Recombinant Anti-Myelin Basic Protein antibody [IGX3421] (ab209328)

Myelin oligodendrocyte glycoprotein (MOG): Recombinant Anti-Myelin oligodendrocyte glycoprotein antibody [EPR4282] (ab108505)

Sox10: Recombinant Anti-SOX10 antibody [EPR4007-104] (ab180862)

Pancreatic cell types

Glucagon: Recombinant Anti-Glucagon antibody [EP3070] (ab92517)

Insulin: Recombinant Anti-Insulin antibody [EPR17359] (ab181547)

Islet-1: Recombinant Anti-Islet 1 antibody [EP4182] (ab109517)

Pancreatic polypeptide: Recombinant Anti-Pancreatic Polypeptide antibody [EPR22853-61] (ab255827)

PDX-1: Recombinant Anti-PDX1 antibody [EPR3358(2)] (ab134150)

Hepatic cell types

α-Fetoprotein: Recombinant Anti-alpha 1 Fetoprotein antibody [EPR9309] (ab169552)

Albumin: Recombinant Anti-Albumin antibody [EPSISR1] (ab137885)

HNF-1α: Recombinant Anti-HNF1 alpha antibody [EPR23054-142] (ab242140)

Tat-SF1: Recombinant Anti-Tat-SF1 antibody [EPR9105(B)] (ab134921)

Cardiac cell types

α-Actinin: Recombinant Anti-Sarcomeric Alpha Actinin antibody [EP2529Y] (ab68167)

Cardiac Troponin I: Recombinant Anti-Cardiac Troponin I antibody [EP1106Y] (ab52862)

Cardiac Troponin T: Recombinant Anti-Cardiac Troponin T antibody [EPR3695] (ab91605)

Myogenin: Recombinant Anti-Myogenin antibody [EPR4789] (ab124800)

Nkx2.5: Recombinant Anti-Nkx2.5 antibody [EPR20168] (ab205263)