Metabolism is a complex process that lies at the core of biology. Metabolic changes can lead to a huge range of outcomes in different disease areas, from cancer or neurodegeneration to obesity and diabetes. Our assay kits enable you to analyze the pathways of metabolism in-depth in live cells, lysates, and biofluids with easy analysis on your plate reader. With 20 years of experience in manufacturing metabolism assay kits, we can help you understand your cell or disease model or how your target or compound affects your metabolic pathway/phenotype of interest.
Glycolysis is the process by which glucose is converted into pyruvate to provide energy to cells. The glycolysis pathway is a sequence of reactions regulated by a few key enzymes including phosphofructokinase 1. Our assay kits allow you to measure the activity of these enzymes as well as the metabolites involved in this pathway.
| Analyte | Recommended assay kit |
| Glucose | Glucose assay kit (ab65333) Glucose uptake assay kit - Colorimetric (ab136955) |
| Hexokinase | Hexokinase activity assay kit - Colorimetric (ab136957) |
| Pyruvate | Pyruvate assay kit (ab65342) |
| Pyruvate Kinase | Pyruvate kinase assay kit (ab83432) |
| Glycolysis | Glycolysis assay (ab197244) |
Browse all glycolysis products
Fatty acid metabolism
Fatty acid metabolism is key to the production and storage of energy. When released from adipocytes, fatty acid circulates as free fatty acid in the blood, before it enters cells and is broken down to produce energy. Our assay kits enable you to study both catabolic and anabolic fatty acid metabolism.
| Analyte | Recommended assay kit |
| Triglyceride | Triglyceride assay kit - Quantification (ab65336) |
| Free fatty acid | Free fatty acid assay kit - Quantification (ab65341) |
| Pyrophsphate | Pyrophosphate assay kit - Colorimetric/fluorometric (ab234040) |
Browse all fatty acid metabolism products
Urea metabolism
The purpose of the urea cycle is to convert waste ammonia into urea for excretion, primarily taking place in the liver. Our assay kits all you to measure the activity of both the metabolites and enzymes involved in the urea cycle.
| Analyte | Recommended assay kit |
| Urea | Urea assay kit (ab83362) |
| Glutamate | Glutamate assay kit (ab83389) |
| L-arginine | L-arginine assay kit (ab241028) Alanine transaminase activity assay kit (ab105134) Aspartate aminotransferase activity assay kit (ab105135) |
Browse all urea metabolism products
Assays for ATP, NADH and other associated molecules
To further support your work across key metabolism pathways, we provide assay kits across a range of associated molecules. All cellular assay kits recommended below have a plate reader readout.
| Molecule | Recommended assay kit |
| ATP | ATP detection assay kit - Luminescent (ab113849) ATP assay kit - Colorimetric/fluorometric (ab83355) |
| ADP/ATP | ADP/ATP ratio assay kit - Bioluminescent (ab65313) |
| Phosphate | Phosphate assay kit - Colorimetric (ab65622) |
| NAD/NADH | NAD/NADH assay kit - Colorimetric (ab65348) |
| NADP/NADPH | NADP/NADPH assay kit - Colorimetric (ab65349) |
| NADPH | NADPH assay kit - Colorimetric (ab186031) |
4/5 Ease of Use – “We used this pyruvate assay to detect pyruvate in cell pellets and the kit was very simple to use and very fast.”
5/5 Ease of Use –“Instructions were easy to follow with good results”.
Customer, ab65342
4/5 Ease of Use – “Awesome Experience. The kits were easy to use. You are going to [get] protocol with packages when they send to you.”
5/5 Ease of Use – “This kit is easy to use and fast, and gives blood triglyceride concentrations in line with what is reported by literature. Our experience gives reproducible results across the different kit lots”.
Customer, ab65336
5/5 Ease of Use – “Easy to follow instructions and good assay results.”
Customer, ab83362
5/5 Ease of Use – “The assay protocol is good, the assay is easy and quick to perform.”
Customer, ab83389