An alternative to conventional dyes.
DyLight® fluorochromes are high-performance fluorescent conjugates, most commonly used in ICC, IHC, ELISA, and flow cytometry. Useful in both single and multi-color experiments, DyLight® dyes express higher fluorescence intensity and photostability than many spectrally similar dyes.
Our catalog includes DyLight® 488, DyLight® 550, DyLight® 594 and DyLight® 650 conjugates, validated extensively in the most popular applications
DyLight® 488
DyLight® 488 is a fluorophore that appears green to human vision. The dye’s maximum excitation/absorption wavelength is 493nm and the maximum emission wavelength is 518nm (see spectra graph, left). Spectrally equivalent dyes include FITC, CY2®, and Alexa Fluor® 488.
DyLight® 550
DyLight® 550 is a fluorophore that appears yellow, with a maximum excitation/absorption wavelength of 562nm and maximum emission wavelength of 576nm (see spectra graph, left). Spectrally equivalent dyes include TRITC, Cy3®, and Alexa Fluor® 555.
DyLight® 594
DyLight® 594 is a fluorophore that appears orange, with a maximum excitation/absorption wavelength of 593nm and maximum emission wavelength of 618nm (see spectra graph, left). Texas Red® is a good spectral equivalent.
DyLight® 650
DyLight® 650 is a fluorophore that appears orange, with a maximum excitation/absorption wavelength of 652nm and maximum emission wavelength of 672nm (see spectra graph, left). Cy5® is a good spectral equivalent.
If working with more than one DyLight® conjugated secondary antibody, it is important to use antibodies with distinct emission maximum (at least 40 nm difference). The emission spectra of each dye can be seen below:
DyLight® is a trademark of Thermo Fisher Scientific Inc. and its subsidiaries.
Cy® and CyDye® are trademarks of GE Healthcare Limited.
Alexa Fluor® is a registered trademark of Life Technologies. Alexa Fluor® dye conjugates contain(s) technology licensed to Abcam by Life Technologies.