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DyLight® conjugated secondary antibodies

DyLight® conjugated secondary antibodies

An alternative to conventional dyes.

DyLight® fluorochromes are high-performance fluorescent conjugates, most commonly used in ICC, IHC, ELISA, and flow cytometry. Useful in both single and multi-color experiments, DyLight® dyes express higher fluorescence intensity and photostability than many spectrally similar dyes.

Our catalog includes DyLight® 488, DyLight​® 550, DyLight​® 594 and DyLight® 650 conjugates, validated extensively in the most popular applications

Find the right DyLight® secondary antibody for you

Last edited Fri 28 Apr 2023

Advantages of DyLight® conjugated secondary antibodies

  • High fluorescent intensity: intense emission provides high sensitivity. 
  • Narrow emission spectra: minimal bleed-through between fluorochrome channels makes multi-color imaging easy.
  • High photostability: higher resistance to photobleaching.
  • pH stability: remains stable and fluorescent in buffers from pH 4.0-9.0.
  • Enables high dye-to-antibody ratio: water solubility prevents precipitation of conjugates. 

Popular DyLight® fluorochromes

DyLight® 488

DyLight® 488 is a fluorophore that appears green to human vision. The dye’s maximum excitation/absorption wavelength is 493nm and the maximum emission wavelength is 518nm (see spectra graph, left). Spectrally equivalent dyes include FITC, CY2®, and Alexa Fluor® 488.

Image 1 (left): DyLight® 488 spectra graph

DyLight® 550

DyLight® 550 is a fluorophore that appears yellow, with a maximum excitation/absorption wavelength of 562nm and maximum emission wavelength of 576nm (see spectra graph, left). Spectrally equivalent dyes include TRITC, Cy3®, and Alexa Fluor® 555.

DyLight® 550

Image 2 (left): DyLight® 550 spectra graph

DyLight® 594

DyLight® 594 is a fluorophore that appears orange, with a maximum excitation/absorption wavelength of 593nm and maximum emission wavelength of 618nm (see spectra graph, left). Texas Red® is a good spectral equivalent.

DyLight® 594

Image 3 (left): DyLight® 594 spectra graph

DyLight® 650

DyLight® 650 is a fluorophore that appears orange, with a maximum excitation/absorption wavelength of 652nm and maximum emission wavelength of 672nm (see spectra graph, left). Cy5® is a good spectral equivalent.

DyLight® 650

Image 4 (left): DyLight® 650 spectra graph

Multiple staining with DyLight® conjugated secondary antibodies

If working with more than one DyLight® conjugated secondary antibody, it is important to use antibodies with distinct emission maximum (at least 40 nm difference). The emission spectra of each dye can be seen below:

Image 5 (above): Emission spectra of DyLight®​ fluorochromes available. Line colors represent the approximate visible colors of the wavelength maxima.

DyLight®​ is a trademark of Thermo Fisher Scientific Inc. and its subsidiaries.

Cy®​ and CyDye® are trademarks of GE Healthcare Limited.

Alexa Fluor® is a registered trademark of Life Technologies. Alexa Fluor® dye conjugates contain(s) technology licensed to Abcam by Life Technologies.