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HRP secondary antibodies

Extensively tested and optimized for diverse applications including WB, IHC and ELISA.

Horseradish peroxidase (HRP) is a rapid and stable enzyme commonly used as a detection reagent in immunoassays such as western blot, immunohistochemistry (IHC), and ELISA. The high turnover rate of HRP enables secondary antibodies conjugated to this enzyme to generate strong signals in short time spans.

HRP substrates can fall into different categories, including chromogenic (eg DAB, TMB, OPD), fluorogenic (eg ADHP), and chemiluminescent (eg ECL) depending on whether they produce a colored, fluorometric, or luminescent derivative.

We offer HRP secondary antibodies against diverse species and their isotypes, as well as in fragment and pre-adsorbed forms.

Find the right HRP secondary antibody for you.

Last edited Fri 28 Apr 2023

Advantages of our range of HRP-conjugated secondary antibodies

  • High sensitivity – detect low-abundant targets with high signal-to-noise ratios due to an optimal number of HRP molecules per antibody

  • Robust – can be used at dilutions ranging from 1/2,000 to 1/20,000 and provide high specificity and minimal background

  • Stable – supplied in a liquid format that is ready to use and can be stored at 4°C.

  • Flexible – compatible with chromogenic, fluorogenic, and chemiluminescent substrates

  • Extensive validation – extensively tested and optimized for WB, IHC, and ELISA


To further minimize background from cross-species reactivity, we recommend using HRP pre-adsorbed secondary antibodies.

Find the right HRP secondary antibody for you.

Our recommended HRP-conjugated secondary antibodies

SpeciesReactivityIsotypeabID
GoatMouseIgGab205719
GoatRabbitIgGab205718
RabbitHumanIgGab6759
GoatDonkeyIgGab205723
GoatRatIgGab205720
GoatChickenIgYab205721