Human stem cell-derived glutamatergic neurons
Discover consistent and scalable human stem cell-derived glutamatergic neurons for research and drug discovery.
ioGlutamatergic Neurons have been generated from human induced pluripotent stem cells (iPSC) by deterministic cell programming using opti-ox technology. Within days, human stem cells convert into mature and functional glutamatergic neurons, providing a high-quality cellular model with simple protocols for the study of neurological activity and disease.
Watch the seven-day time course, courtesy of bit.bio, which captures the rapid morphological changes from iPSCs upon programming: https://share.vidyard.com/watch/zHAc1Jvg5ypmxGaWMmtggm.
The video shows early neurons reaching the post-mitotic stage by day four and starting to build a dense neural network.
Highly characterized and defined cell identity
ioGlutamatergic Neurons are characterized by immunocytochemistry, gene expression, and bulk RNAseq. ioGlutamatergic Neurons cultures consist mainly of glutamatergic neurons (>80%) characterized by homogenous expression of pan-neuronal proteins MAP2 and TUBB3, and glutamate transporter genes VGLUT1 and VGLUT2. The minor remaining fraction of the neuronal population express marker genes of cholinergic neurons.
Functional ioGlutamatergic Neurons display neuronal activity that matures over time
Accelerated in vitro target and drug discovery using ioGlutamatergic Neurons
Although iPSC-derived neurons have promising applications in drug discovery platforms, their use within high throughput screening (HTS) has previously been limited by traditional ‘directed differentiation’ approaches, making it difficult to generate reliable, homogenous cell populations at scale. Human iPSC-derived ioGlutamatergic Neurons overcome these restrictions to provide a robust platform for drug discovery, showing good suitability for high-throughput screening in 384-well plates.
Watch Dr. Shushat Jain, Group Leader for In vitro Biology, Discovery, Charles River on ioGlutamatergic Neurons: https://share.vidyard.com/watch/kig8nr4z4aQeWbc31nDMwG
Ready-to-culture glutamatergic neurons provide:
Ease of Use: Cells arrive programmed to rapidly mature upon revival with only one medium required in a two-step protocol.
Consistency: Batch-to-batch reproducibility and homogeneity create a stable human model for excitatory neuronal activity and disease.
Speed: Ready for experimentation as early as two days post revival and allow the formation of functional neuronal networks at 17 days.
Scalability: Industrial scale quantities allow the cells to be used in a range of applications, from research to screening purposes.