Matched antibody pairs for ELISA
Create your own sandwich ELISA with sensitive and consistent monoclonal matched antibody pairs. Available as kits or PBS-only pairs for more flexibility.
Matched antibody pairs for ELISA
Our matched antibody pairs come in two formats:
- Carrier-free antibody pairs (BSA, glycerol, and azide free): include a capture and detector antibody pair, suitable for ELISA-based assays and available as 10 x 96-well plate. To enable an easy scale-up of your ELISA assay, individual capture and detector antibodies can also be purchased separately in 100 µg and 1 mg sizes.
- Antibody pair kits: include a titrated capture and biotinylated detector antibody pair and a calibrated protein standard. Available in two sizes, with enough reagents for either 5 or 10 x 96-well plates using a standard sandwich ELISA.
Browse our range of matched antibody pairs.
Our carrier-free matched antibody pairs and kits deliver consistent, specific, and sensitive results.
- Batch-to-batch consistency: onlyrecombinantmonoclonal antibodies are used in our matched antibody pairs.
- Specificity: matched antibody pairs are screened in plasma and serum to ensure specificity in complex samples.
- Sensitivity: benchmarked against commercially available antibody pairs to ensure equivalent or superior performance compared with the competition.
- Flexibility: Our carrier-free matched antibody pairs offer even more flexibility in terms of conjugation and assay setup.
Learn more about the advantages of carrier-free antibodies and antibody pairs
High-throughput screening (HTS) and matched antibody pairs
Getting the most out of your HTS and drug discovery requires optimal assay development to generate the largest volume of data in the shortest possible time. Our matched antibody pairs are ideal for tailoring your ELISA assays to your own plate systems as they can be used in 96 or 384-well formats to scale up your HTS. Our antibody pairs are extremely flexible; they can be used with many different platforms and run using standard plate readers.
In addition to ELISA assays, our matched antibody pairs can be used in other assays adapted to high-throughput scale such as fluorescent proximity assays (TR-FRET: Time-Resolved Fluorescence Energy Transfer). With over 1,300 carrier-free antibody pairs and over 200 matched antibody pair kits, you get immediate access to a huge variety of antibody pairs in an entirely customizable format. The range of carrier-free pairs on our catalog is also growing every year to give you access to the target you need. The capture and detector antibodies can also be purchased separately for extra flexibility in designing and scaling up your assay.
Figure 1. The human IL-1Ra matched antibody pair kit (ab210888) is 10-fold more sensitive than a rabbit polyclonal antibody pair when measuring human IL-1Ra standard protein using a sandwich ELISA.
Protein standards
Protein standards are essential references and tools used to generate a standard curve and quantify the concentration of the target protein in a sample.
To support your immunoassay development, we offer a growing range of recombinant protein standards complementary with our matched antibody pairs, capture and detector antibodies and SimpleStep ELISA kit range.
These are the same proteins used to develop our antibody pairs, and are available as 10 µg, characterized and validated with SDS-PAGE and sandwich ELISA using the corresponding capture and detector antibodies.