Pre-adsorbed secondary antibodies

Minimize non-specific binding and high background staining.

Last edited Mon, 14 Apr 2025

Explore our best-selling pre-adsorbed secondary antibody

Pre-adsorption (also referred to as cross-adsorption) is an extra purification step introduced to increase the specificity of a secondary antibody. The solution containing secondary antibodies is passed through a column matrix containing immobilized serum proteins from potentially cross reactive species. Non-specific secondary antibodies are retained in the column, while highly specific secondaries flow through.

Advantages of pre-adsorbed secondary antibodies

We always recommend using pre-adsorbed (cross-absorbed) secondary antibodies to minimize non-specific background when working with tissues and cells. This means pre-adsorbed antibodies are useful in many different experiments. However, they are most useful for:

Multicolor experiments: When several primaries and their corresponding secondary antibodies are used simultaneously, the secondary antibody should be pre-adsorbed against the species in which the other primary antibodies have been raised to reduce the risk of cross-reactivity.
- Example: Use an anti-rabbit Alexa Fluor® 488 pre-adsorbed secondary against mouse serum and an anti-mouse Alexa Fluor® 647 pre-adsorbed secondary against rabbit serum when performing a double staining experiment using primary antibodies raised in rabbit and mouse, respectively.
IHC experiments on samples with abundant amounts of endogenous immunoglobulins (Igs):The secondary antibody should be pre-adsorbed against the same species the sample originated from to reduce the risk of cross-reactivity between the secondary antibody and endogenous Igs.
- Example: Use an anti-mouse HRP pre-adsorbed secondary antibody against human serum when staining human tissues or cell lines.

Find the right pre-adsorbed secondary antibody

Pre-adsorbed Alexa Fluor® conjugated secondary antibodies

All pre-adsorbed Alexa Fluor® secondary antibodies listed below are suitable for ICC/IF, ELISA, IHC-P, IHC-Fr and flow cytometry.

Conjugate

Product name

Alexa Fluor® 405

Anti-rabbit Alexa Fluor® 405 (IgG H&L) preadsorbed (ab175648)

Anti-rat Alexa Fluor® 405(IgG H&L) preadsorbed (ab175670)

Anti-goat Alexa Fluor® 405 (IgG H&L) preadsorbed (ab175665)

Anti-chicken Alexa Fluor® 405(IgY H&L) preadsorbed (ab175675)

Alexa Fluor® 488

Anti-mouse Alexa Fluor® 488 (IgG H&L) preadsorbed (ab150117)

Anti-rabbit Alexa Fluor® 488 (IgG H&L) preadsorbed (ab150081)

Anti-rat Alexa Fluor® 488 (IgG H&L) preadsorbed (ab150153)

Anti-goat Alexa Fluor® 488 (IgG H&L) preadsorbed (ab150133)

Anti-chicken Alexa Fluor® 488 (IgY H&L) preadsorbed (ab150173)

Alexa Fluor® 555

Anti-mouse Alexa Fluor® 555 (IgG H&L) (ab150110)

Anti-rabbit Alexa Fluor® 555 (IgG H&L) (ab150086)

Anti-rat Alexa Fluor® 555 (IgG H&L) (ab150154)

Anti-goat Alexa Fluor® 555 (IgG H&L) (ab150134)

Anti-chicken Alexa Fluor® 555 (IgY H&L) (ab150174)

Alexa Fluor® 594

Anti-mouse Alexa Fluor® 594 (IgG H&L) (ab150120)

Anti-rabbit Alexa Fluor® 594 (IgG H&L) (ab150064)

Anti-rat Alexa Fluor® 594 (IgG H&L) (ab150156)

Anti-goat Alexa Fluor® 594 (IgG H&L) (ab150136)

Anti-chicken Alexa Fluor® 594 (IgY H&L) (ab150176)

Alexa Fluor® 647

Anti-mouse Alexa Fluor® 647 (IgG H&L) (ab150119)

Anti-rabbit Alexa Fluor® 647 (IgG H&L) (ab150083)

Anti-rat Alexa Fluor® 647 (IgG H&L) (ab150155)

Anti-goat Alexa Fluor® 647 (IgG H&L) (ab150135)

Anti-chicken Alexa Fluor® 647 (IgY H&L) (ab150175)

Pre-adsorbed HRP conjugated secondary antibodies

Please note some of our pre-adsorbed HRP secondary antibodies are only suitable for use in specific applications, these are listed below.

Product Name

Applications

Anti-mouse HRP (IgG H&L) (ab97040)

IHC-P, ELISA, WB

Anti-rabbit HRP (IgG H&L) (ab97080)

ICC, IHC-P, ELISA, WB

Anti-rat HRP (IgG H&L) (ab7097)

ICC/IF, IHC-P, IHC-Fr, WB, ELISA, Dot Blot

Anti-goat HRP (IgG H&L) (ab97120)

ICC, IHC-P, ELISA, WB

Anti-chicken HRP (IgY H&L) (ab7118)

WB, Immunomicroscopy, ICC/IF, Dot Blot, ELISA, IHC-P, IHC-Fr

Pre-adsorbed biotinylated secondary antibodies

Please note some of our pre-adsorbed biotinylated secondary antibodies are only suitable for use in specific applications. These are listed below

Product Name

Applications

Anti-mouse Biotin (IgG H&L) (ab7074)

ICC/IF, Dot Blot, ELISA, IHC-P, IHC-Fr, Immunomicroscopy, WB

Anti-rabbit Biotin (IgG H&L) (ab7082)

ICC/IF, WB, ELISA, Immunomicroscopy, Dot Blot, IHC-P, IHC-Fr

Anti-rat Biotin (IgG H&L) (ab7096)

ICC/IF, WB, ELISA, Immunomicroscopy, Dot Blot, IHC-P, IHC-Fr

Anti-goat Biotin (IgG H&L) (ab7124)

ICC/IF, Dot Blot, ELISA, IHC-P, IHC-Fr, Immunomicroscopy, WB

Anti-chicken Biotin (IgY H&L) (ab7117)

ICC/IF, Dot Blot, ELISA, IHC-P, IHC-Fr, Immunomicroscopy, WB

Alexa Fluor® is a registered trademark of Life Technologies. Alexa Fluor® dye conjugates contain(s) technology licensed to Abcam by Life Technologies.