You can use different inhibitor combinations for your experiment, as well as water soluble & DMSO versions. Browse the guide below for more information.
Cell lysates contain endogenous proteases which degrade proteins and can affect sensitivity and reliability in protein expression research. It is therefore common practice to add protease inhibitors to lysate samples to protect proteins of interest. Our protease inhibitor cocktails inhibit a number of proteases including serine, cysteine and aspartic proteases.
Kit name
Key information
Inhibitors included
Target protease/phosphatase
Optimized to generate the maximal protection to mammalian tissue and cell extracts.
Aprotinin, Leupeptin, Pepstain A, PMSF
Acidic protease, Cysteine protease, Mammalian acetylcholinesterase, Serine protease
Ideal for mamalian cell/tissue lysates and bacterial cell extracts using metal chelation chromatography
AEBSF hydrochloride, Aprotinin (bovine lung), Bestatin, E-64, Leupeptin hemisulfate, Pepstatin A
Aminopeptidase B and leucine aminopeptidase, Aspartic protease, Cysteine protease, Serine protease
General use cocktail
AEBSF hydrochloride, Aprotinin (bovine lung), E-64, EDTA disodium salt, Leupeptin hemisulfate
Cysteine protease, Metalloprotease, Serine protease
Ideal for bacterial cell extracts using metal chelation chromatography
AEBSF hydrochloride, Bestatin, E-64, EDTA disodium salt, Pepstatin A,
Aminopeptidase B and leucine aminopeptidase, Aspartic protease, Cysteine protease, Metalloprotease, Serine protease
Ideal for fungal and yeast cell extracts
AEBSF hydrochloride, E-64, Pepstatin A, o-Phenanthroline
Aspartic protease, Cysteine protease, Metalloprotease, Serine protease
Approximately 30% of known proteins can be reversibly phosphorylated in order to control a number of cellular processes. Protein kinases predominantly phosphorylate at serine, threonine and tyrosine residues - phosphatases reverse this phosphorylation. When studying these processes and pathways it is therefore essential to preserve the phosphorylation state of these proteins in your samples. Our phosphatase inhibitor cocktails inhibit a number of phosphatases including specific serine/threonine and tyrosine phosphatases as well as non-specific acid and alkaline phosphatases.
Kit name
Inhibitors included
Target protease/phosphatase
β-Glycerophosphate, Sodium fluoride, Sodium orthovanadate, Sodium pyrophosphate decahydrate
Acid phosphatase, Alkaline phosphatase, Protein tyrosine phosphatase, Serine/threonine phosphatase
Imidazole, Sodium fluoride, Sodium molybdate, Sodium orthovanadate, Sodium tartrate dihydrate
Acid phosphatase, Alkaline phosphatase, Protein tyrosine phosphatase, Serine/threonine phosphatase
(-)-p-Bromotetramisole oxalate, Cantharidin, Calyculin A
Alkaline Phosphatase, Serine/threonine Phosphatase (PP1 and PP2A)
β-Glycerophosphate, Imidazole, Sodium fluoride, Sodium molybdate, Sodium orthovanadate, Sodium tartrate dihydrate
Acid Phosphatase, Alkaline phosphatase, Protein tyrosine phosphatase, Serine/threonine phosphatase
Combined protease and phosphatase inhibitor cocktail. Ideal for the ultimate protection of your protein samples. Available with and without EDTA, a metalloproteinase inhibitor.
Kit name
Key information
Inhibitors included
Target protease/phosphatase
AEBSF-free and mass spectroscopy compatible - water soluble
Aprotinin (bovine lung), Bestatin, E-64, Leupeptin hemisulfate, β-Glycerophosphate, Sodium fluoride, Sodium orthovanadate, Sodium pyrophosphate decahydrate, EDTA disodium salt
Aminopeptidase B and leucine aminopeptidase, Cysteine protease, Metalloprotease, Serine protease, Acid phosphatase, Alkaline phosphatase, Protein tyrosine phosphatase, Serine/threonine phosphatase
AEBSF-free and mass spectroscopy compatible - water soluble
Aprotinin (bovine lung), Bestatin, E-64, Leupeptin hemisulfate, β-Glycerophosphate, Sodium fluoride, Sodium orthovanadate, Sodium pyrophosphate decahydrate
Aminopeptidase B and leucine aminopeptidase, Cysteine protease, Serine protease, Acid phosphatase, Alkaline phosphatase, Protein tyrosine phosphatase, Serine/threonine phosphatase