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Protease and phoshatase inhibitor cocktails

Protect your protein samples from degradation and preserve their phosphorylation state with our convenient and easy-to-use protease and phosphatase inhibitor cocktails.

Explore our best-selling protease inhibitor

​You can use different inhibitor combinations for your experiment, as well as water soluble & DMSO versions. Browse the guide below for more information.​

Protease inhibitor cocktails

Phosphatase inhibitor cocktails

Protease and phosphatase inhibitor cocktails

Last edited Thu 26 Nov 2020

Protease inhibitor cocktails

Cell lysates contain endogenous proteases which degrade proteins and can affect sensitivity and reliability in protein expression research. It is therefore common practice to add protease inhibitors to lysate samples to protect proteins of interest. Our protease inhibitor cocktails inhibit a number of proteases including serine, cysteine and aspartic proteases. 

Kit nameKey informationInhibitors includedTarget protease/phosphatase

Protease Inhibitor Cocktail

Optimized to generate the maximal protection to mammalian tissue and cell extracts.Aprotinin, Leupeptin, Pepstain A, PMSFAcidic protease, Cysteine protease, Mammalian acetylcholinesterase, Serine protease
Protease Inhibitor Cocktail (EDTA-free)Ideal for mamalian cell/tissue lysates and bacterial cell extracts using metal chelation chromatography AEBSF hydrochloride, Aprotinin (bovine lung), Bestatin, E-64, Leupeptin hemisulfate, Pepstatin AAminopeptidase B and leucine aminopeptidase,  Aspartic protease, Cysteine protease, Serine protease
Protease Inhibitor Cocktail IIGeneral use cocktailAEBSF hydrochloride, Aprotinin (bovine lung), E-64, EDTA disodium salt, Leupeptin hemisulfateCysteine protease, Metalloprotease, Serine protease
Protease Inhibitor Cocktail IIIIdeal for bacterial cell extracts using metal chelation chromatography AEBSF hydrochloride, Bestatin, E-64, EDTA disodium salt, Pepstatin A, Aminopeptidase B and leucine aminopeptidase, Aspartic protease, Cysteine protease, Metalloprotease,  Serine protease
Protease Inhibitor Cocktail IVIdeal for fungal and yeast cell extractsAEBSF hydrochloride, E-64, Pepstatin A, o-PhenanthrolineAspartic protease, Cysteine protease, Metalloprotease, Serine protease

Phosphatase inhibitor cocktails

Approximately 30% of known proteins can be reversibly phosphorylated in order to control a number of cellular processes. Protein kinases predominantly phosphorylate at serine, threonine and tyrosine residues - phosphatases reverse this phosphorylation. When studying these processes and pathways it is therefore essential to preserve the phosphorylation state of these proteins in your samples. Our phosphatase inhibitor cocktails inhibit a number of phosphatases including specific serine/threonine and tyrosine phosphatases as well as non-specific acid and alkaline phosphatases.

Kit nameInhibitors includedTarget protease/phosphatase
Phosphatase Inhibitor Cocktail I β-Glycerophosphate, Sodium fluoride, Sodium orthovanadate, Sodium pyrophosphate decahydrateAcid phosphatase, Alkaline phosphatase, Protein tyrosine phosphatase, Serine/threonine phosphatase
Phosphatase Inhibitor Cocktail IIImidazole, Sodium fluoride, Sodium molybdate, Sodium orthovanadate, Sodium tartrate dihydrateAcid phosphatase, Alkaline phosphatase, Protein tyrosine phosphatase, Serine/threonine phosphatase
Phosphatase Inhibitor Cocktail III(-)-p-Bromotetramisole oxalate, Cantharidin, Calyculin AAlkaline Phosphatase, Serine/threonine Phosphatase (PP1 and PP2A)
Phosphatase Inhibitor Cocktail IVβ-Glycerophosphate, Imidazole, Sodium fluoride, Sodium molybdate, Sodium orthovanadate, Sodium tartrate dihydrateAcid Phosphatase, Alkaline phosphatase, Protein tyrosine phosphatase, Serine/threonine phosphatase

Protease and phosphatase inhibitor cocktails

Combined protease and phosphatase inhibitor cocktail. Ideal for the ultimate protection of your protein samples. Available with and without EDTA, a metalloproteinase inhibitor.

Kit nameKey informationInhibitors includedTarget protease/phosphatase
Protease and Phosphatase Inhibitor CocktailAEBSF-free and mass spectroscopy compatible - water solubleAprotinin (bovine lung), Bestatin, E-64, Leupeptin hemisulfate, β-Glycerophosphate, Sodium fluoride, Sodium orthovanadate, Sodium pyrophosphate decahydrate, EDTA disodium saltAminopeptidase B and leucine aminopeptidase, Cysteine protease, Metalloprotease, Serine protease, Acid phosphatase, Alkaline phosphatase, Protein tyrosine phosphatase, Serine/threonine phosphatase
Protease and Phosphatase Inhibitor Cocktail (EDTA-free)AEBSF-free and mass spectroscopy compatible - water solubleAprotinin (bovine lung), Bestatin, E-64, Leupeptin hemisulfate, β-Glycerophosphate, Sodium fluoride, Sodium orthovanadate, Sodium pyrophosphate decahydrateAminopeptidase B and leucine aminopeptidase, Cysteine protease, Serine protease, Acid phosphatase, Alkaline phosphatase, Protein tyrosine phosphatase, Serine/threonine phosphatase