Sample Prep & Detection Kits
Conjugation kitsPurification kitsSample preparation kitsChromogen kitsIHC kitsChIP kitsAccessory Reagents & Controls
Accessory reagents & controlsBiochemicals
BiochemicalsProteins and Peptides
Proteins and peptidesAnti-Ly6g antibody [1A8] - mouse IgG2c (Chimeric)
Low endotoxin, Azide free.
Our first-to-market chimera with mouse IgG2c backbone, this functional antibody specifically depletes neutrophils in vivo for up to 72h.
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IHC reagents
Equipment
Perform deparaffinization and rehydration on Leica ST5020 Multistainer using the following program.
Perform antigen retrieval on Biocare Medical Decloaking Chamber™ Plus using the following setting.
Cool slide at room temperature for 15 min.
IHC reagents
Equipment
Set up slides on BioGenex i6000 Autostainer.
Apply the peroxidase block for 10 min.
Wash 3 times with wash buffer.
Apply the protein block to the slides and incubate 20 min at room temperature.
Wash 3 times with wash buffer.
Apply the pre-diluted antibodies to the slides (see Materials) and incubate for 1 hour at room temperature.
Wash 3 xtimes with wash buffer.
Add the post primary block (NovoLink Kit) to the slides and incubate for 30 min.
Wash 3 times with IHC wash buffer.
Add the NovoLink polymer (NovoLink Kit) to the slides and incubate for 45 min.
Rinse 3 times with wash buffer.
Add the DAB chromogen substrate and develop for 5 min.
Wash slides 5 times with dH2O at room temperature.
Counterstain with hematoxylin (Novolink kit) for 1 min at room temperature.
Wash slides 5 times with dH2O at room temperature.
IHC reagents
Equipment
Remove the slides from BioGenex i6000 Autostainer.
Set up slides on Leica Multistainer ST5020.
Dehydrate using ethanol.
Wash with xylene, 3 times for 2 min.
Coverslip with Cytoseal Mounting Medium in the Leica Auto Coverslipper CV5030.