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Proteins and peptidesAnti-Ly6g antibody [1A8] - mouse IgG2c (Chimeric)
Low endotoxin, Azide free.
Our first-to-market chimera with mouse IgG2c backbone, this functional antibody specifically depletes neutrophils in vivo for up to 72h.
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Apoptosis may be induced in experimental systems through a variety of methods. In general, methods to induce apoptosis can be divided into two categories: biological induction and chemical induction.
Activation of Fas or TNF receptors by their respective ligands, or by cross-linking with an agonist antibody, induces apoptosis of Fas- or TNF receptor-bearing cells. Here we describe a general protocol to induce apoptosis using an anti-Fas receptor (anti-CD95) monoclonal antibody (mAb) in Jurkat cells. Our mouse monoclonal anti-Fas antibody [UT-1] (ab11881) can be used for apoptosis indication.
Grow Jurkat cells in RPMI-1640 containing 10% fetal bovine serum (FBS) in a humidified 5% CO2 incubator at 37°C.
Harvest exponentially growing cells at a concentration of 105 cells/mL by centrifugation at 300–350 x g for 5 min.
Add anti-Fas (anti-CD95) mAb to the appropriate concentration. For ab11881, use a final concentration of 2–20 mg/mL.
For a negative control, incubate untreated cells (without anti-Fas/CD95 antibody) under the same conditions.
Harvest the cells by centrifugation at 300–350 x g for 5 min.
Remove all medium and resuspend cells in PBS.
Repeat centrifugation step and resuspend cells.
Proceed to detect apoptosis using your method of choice.
Collect cells and detect apoptosis using your method of choice.