Antibodies against specific organelles, the cell membrane, or cytoskeletal components allow you to explore protein localization  in situ.  Also,you can use them in western blot analyses to confirm the proper fractionation of cell lysates.

Microtubules

Microtubules:  highly dynamic polymers composed of 13 protofilaments of α-tubulin and β-tubulin heterodimers that continuously grow and shrink during interphase and mitosis.

We recommend

Anti-alpha Tubulin antibody [EP1332Y] - Microtubule Marker (ab52866)

Anti-beta Tubulin antibody [EPR16774] (ab179513)

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Vimentin

Vimentin:  class-III intermediate filaments found in various non-epithelial cells, especially mesenchymal cells. Vimentin is attached to the nucleus, endoplasmic reticulum, and mitochondria, either laterally or terminally.

We recommend

Anti-Vimentin antibody [EPR3776] - Cytoskeleton Marker (ab92547)

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Desmin

Desmin:  class-III intermediate filaments found in muscle cells. In adult striated muscle, they form a fibrous network connecting myofibrils to each other and the plasma membrane from the periphery of the Z-line structures.

We recommend

Anti-Desmin antibody [Y66] - Cytoskeleton Marker (ab32362)

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Cytokeratin

Cytokeratin:  intermediate filaments present in all epithelial cells and several non-epithelial cells. These may regulate the activity of kinases, such as PKC and SRC, via binding to integrin beta-1 (ITB1) and the receptor of activated protein kinase C (RACK1/GNB2L1).

We recommend

Anti-Cytokeratin 19 antibody [EP1580Y] - Cytoskeleton Marker (ab52625)

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