Granulocyte markers

Your guide to identifying and studying neutrophils, basophils, and eosinophils.

Granulocytes are actors in the innate immune system and the body’s first line of defense against pathogens.

Cell
Function
Markers
Neutrophil
Their function is achieved by the processes of phagocytosis, degranulation, neutrophilic extracellular trap (NET) formation (NETosis), and oxidative burst
CD11b, CD14(-), CD15, CD54
Basophil
Primary effector cells in allergy processes
CD45, CD123, CD294
Eosinophil
Generate inflammatory responses locally and systemically against invading parasites, fungi and even viruses, through the release of cytotoxic granules
CD49d, CD66b, CD125, CD193

CD11b

Alternative names Integrin alpha-M, CR3A, ITGAM, MAC-1

Regulates macrophage polarization, inhibits immune suppression, modulates neovascularization and cancer.

We recommend

Anti-CD11b antibody [M1/70] (ab8878)

Anti-CD11b antibody [ICRF44] (ab34216)

CD14

CD14 is used as a negative expression marker to distinguish neutrophils from other cell types.

We recommend

Anti-CD14 antibody [SP192] (ab183322)

CD15

Alternative names ELFT, FCT3A, FUC-TIV

Cell adhesion. Mediates phagocytosis and chemotaxis. Involved in leukocyte homing, and adhesion of cancer cells to brain endothelium. Mediates neutrophil adhesion to dendritic cells.

We recommend

Anti-CD15 antibody [SP159] (ab135377)

CD45

Alternative names Leukocyte common antigen, L-CA, PTPRC

Critical for B- and T-cell receptor-mediated activation. Also required for thymic selection.

We recommend

Anti-CD45 antibody [EPR27167-58] (ab303670)

CD49d

Alternative names Integrin alpha-4, VLA-4 subunit alpha, ITGA4

Involved with retention and release of neutrophils. Critical role in leukocyte trafficking, activation and survival.

CD54

Alternative names ICAM1, BB2

Extravasation of leukocytes from blood vessels. Regulates T-cell activation. Involved in tumorigenesis of various cancers.

We recommend

Anti-ICAM1 antibody [EPR24639-3] (ab282575)

CD66b

Alternative names CEACAM8, CD67, CGM6, NCA-95

Cell adhesion. Superoxide production. Degranulation of eosinophils. Neutrophil activation.

We recommend

Anti-CD66b antibody [GM-2H6] (ab48589)

CD123

Alternative names IL3RA

Receptor for IL3. Associates with the GM-CSF receptor. Involved in normal cell development.

We recommend

Browse all CD123 antibodies

CD125

Alternative names IL5RA, IL5R

Receptor for IL5. Involved in suppression of ROS formation. Promotes growth and differentiation of eosinophil precursors. Activates mature eosinophils.

We recommend

Browse all CD125 antibodies

CD193

Alternative names CCR3, CKR3, CMKBR3

Receptor for several chemokines that may contribute to accumulation and activation of eosinophils and other inflammatory cells in the allergic airway. Entry co-receptor for HIV-1.

We recommend

Alexa Fluor® 488 Anti-CCR3 antibody [5E8] (ab270656)

CD294

Alternative names GPR44, CRTH2

Involved in chemotaxis and eosinophil migration.

We recommend

Browse all CD294 antibodies

Further reading

See the references below for a list of relevant review.

References

View 4 references for Further reading

References

Further reading

Wood, B. Multicolor Immunophenotyping: Human Immune System Hematopoiesis  Methods Cell Biol.  75 ,559-576 (2004)

Elghetany, M., Elghetany, M. Surface Antigen Changes during Normal Neutrophilic Development: A Critical Review  Blood Cells, Mol. Dis.  28 ,260-274 (2002)

Mantovani, A., Cassatella, M. A., Constantini, C., Jaillon, S. Neutrophils in the activation and regulation of innate and adaptive immunity  Nat. Rev. Immunol.   11 ,519-531 (2011)

Behnen, M., et al. Immobilized Immune Complexes Induce Neutrophil Extracellular Trap Release by Human Neutrophil Granulocytes via Fc RIIIB and Mac-1. J  Immunol.   193 ,1954-1965 (2014)