Hypoxia markers
Find the products you need to detect hypoxia using protein markers such as HIF-1a and BNIP3.
HIF-1a
Under hypoxia, HIF-1a is stabilized and moves into the nucleus, where it regulates transcription of genes whose protein products increase oxygen delivery or facilitate metabolic adaptation to hypoxia.
HIF-1a staining of HeLa cells. Anti-HIF-1a antibody [EPR16897] (ab179483) was used at 1/50 dilution with goat anti-rabbit IgG (Alexa Fluor® 488) secondary antibody. Cells were treated with 0.6 nM CoCl2 for 6 hours.
We recommend
HIF-1a transcription factor assay kit (ab133104)
Anti-HIF-1a antibody (ab179483)
Human SimpleStep ELISA® kit (ab171577)
Recombinant human HIF-1a protein (ab154478)
BNIP3
BNIP3 induction by HIF-1α under hypoxic conditions is involved in hypoxia-mediated autophagy, cell survival and tumor progression.
BNIP3 staining of laryngeal squamous cell carcinoma tissue. Anti-BNIP3 mouse monoclonal antibody (ab10433) was used at 1/100 dilution and visualized by DAB.
We recommend
Anti-BNIP antibody [ANa40] (ab10433)
Human HIF-1a + BNIP3 hypoxia in cell ELISA kit (ab129733)
HIF-1a + BNIP3 hypoxia response human flow cytometry kit (ab126585)
References
View 1 reference for BNIP3
PDK1
PDK1 (pyruvate dehydrogenase kinase 1) is downstream of HIF-1α and is induced under hypoxia. Expression during hypoxia channels glucose through glycolysis to maintain ATP production.
PDK1 staining of NIH/3T3 cells. Anti-mitochondrial pyruvate dehydrogenase kinase 1 antibody (ab202468) was used at a dilution of 1/1000 with goat anti-rabbit IgG (Alexa Fluor 488) secondary antibody (ab150077). Cells were counterstained with DAPI (blue) and tubulin also stained (red).
We recommend
Human HIF-1a + PDK1 hypoxia in cell ELISA kit (ab125299)
Anti-mitochondrial pyruvate dehydrogenase kinase 1 antibody (ab202468)
HIF-1a + PDK1 hypoxia response human flow cytometry kit (ab126700)
Recombinant human mitochondrial pyruvate dehydrogenase kinase 1 protein (ab125580)
References
View 1 reference for PDK1
GLUT1
Expression of the GLUT1 glucose transporter is increased under hypoxic conditions, induced by HIF-1α3.
GLUT1 staining of HEPg2 cells. Anti-glucose transporter GLUT antibody (ab115730) was used at a dilution of 1/100 with Alexa Fluor® 488 goat anti-rabbit secondary antibody (ab150077). Cells were counterstained with DAPI and tubulin was also stained (red).
We recommend
Anti-glucose transporter GLUT antibody (ab115730)
HIF-1a + GLUT1 hypoxia response human flow cytometry kit (ab126584)
References
View 1 reference for GLUT1