What are the neuroepithelial cells?

Neuroepithelial (NE) cells are symmetrically dividing cells that form the neural plate and neural tube during embryonic development. They exhibit typical epithelial features such as tight junctions and are highly polarized along their apical-basal axis.

Nestin

Gene name NES

An intermediate filament protein expressed in NE cells. Its expression persists in radial glia until astrocyte development.

iPSCs stained with anti-nestin (red) (ab105389).

We recommend

Nestin antibodies

References

View 2 references for Nestin

SOX2

Alternative names ANOP3, MCOPS3, SRY-box 2, Sox2, SRY-box transcription factor 2

Gene name SOX2

A transcription factor and the earliest marker of the neural plate. It is expressed in proliferating cells and those acquiring glial fates but downregulated in post-mitotic neurons.

E13 mouse spinal cord sections stained with anti-SOX2 (ab97959).

We recommend

SOX2 antibodies

References

View 2 references for SOX2

Notch1

Alternative names Neurogenic locus notch homolog protein 1

Gene name NOTCH1

A transmembrane receptor that regulates the formation, migration, and differentiation of neural crest cells.

Mouse embryo tissue sections stained with anti-notch1 (green) (ab65297).

We recommend

Notch1 antibodies

References

View 2 references for Notch1

HES1 and HES3

Alternative names Hairy and enhancer of split-1 and hairy and enhancer of split-3

Gene name HES1 and HES3

These transcription factors maintain the symmetrical cell division of NE cells. When NE cells become radial glia, HES3 is downregulated and HES5 is upregulated.

Mouse NIH3T3 cells stained with anti-HES1 (green) (ab71559).

We recommend

HES1 antibodies

HES5 antibodies

References

View 1 reference for HES1 and HES3

Occludin

Gene name OCLN

A tight junction protein lost after neural tube closure before neurogenesis.

Human breast cancer cells stained with anti-occludin (red) (ab31721).

We recommend

Occludin antibodies

References

View 2 references for Occludin

E-cadherin

Alternative names epithelial cadherin

Gene name CDH1

Cell adhesion molecule downregulated after neural tube closure before neurogenesis.

Human pluripotent stem cells stained with anti-E-cadherin (ab40772).

We recommend

E-cadherin antibodies

References

View 2 references for E-cadherin

SOX10

Alternative names SRY-box 10, SRY-box transcription factor 10

Gene name SOX10

A transcription factor present in migrating neural crest cells.

A375 cells stained with anti-SOX10 (ab155279).

We recommend

SOX10 antibodies

References

View 4 references for SOX10

References

Nestin

Jessen KR,, Mirsky R. The origin and development of glial cells in peripheral nerves. Nat Rev Neurosci. 6 (9),671-82 (2005)

Kriegstein AR,, Götz M. Radial glia diversity: a matter of cell fate. Glia 43 (1),37-43 (2003)

SOX2

Bylund M,, Andersson E,, Novitch BG,, et al. Vertebrate neurogenesis is counteracted by Sox1-3 activity Nat Neurosci 6 (11),1162-8 (2003)

Papanayotou C,, Mey A,, Birot AM,, et al. A mechanism regulating the onset of Sox2 expression in the embryonic neural plate. PLoS Biol 6 (1), (2008)

Notch1

Krämer A,, Mentrup T,, Kleizen B,, et al. Small molecules intercept Notch signaling and the early secretory pathway. Nat Chem Biol. 9 (11),731-8 (2013)

Noisa P,, Lund C,, Kanduri K,, et al. Notch signaling regulates the differentiation of neural crest from human pluripotent stem cells. J Cell Sci. 127 (9),2083–2094 (2014)

HES1 and HES3

Kageyama R,, Ohtsuka T,, Kobayashi T. Roles of Hes genes in neural development. Dev Growth Differ. 50 (S1),S97-S103 (2008)

Occludin

Jessen KR,, Mirsky R. The origin and development of glial cells in peripheral nerves. Nat Rev Neurosci. 6 (9),671-82 (2005)

Kriegstein AR,, Götz M. Radial glia diversity: a matter of cell fate. Glia 43 (1),37-43 (2003)

E-cadherin

Jessen KR,, Mirsky R. The origin and development of glial cells in peripheral nerves. Nat Rev Neurosci. 6 (9),671-82 (2005)

Kriegstein AR,, Götz M. Radial glia diversity: a matter of cell fate. Glia 43 (1),37-43 (2003)

SOX10

Bylund M,, Andersson E,, Novitch BG,, et al. Vertebrate neurogenesis is counteracted by Sox1-3 activity Nat Neurosci 6 (11),1162-8 (2003)

Jessen KR,, Mirsky R. The origin and development of glial cells in peripheral nerves. Nat Rev Neurosci. 6 (9),671-82 (2005)

Kriegstein AR,, Götz M. Radial glia diversity: a matter of cell fate. Glia 43 (1),37-43 (2003)

Papanayotou C,, Mey A,, Birot AM,, et al. A mechanism regulating the onset of Sox2 expression in the embryonic neural plate. PLoS Biol 6 (1), (2008)