Nuclear markers
Your guide to selecting nuclear markers.
Antibodies against specific organelles, the cell membrane, or cytoskeletal components allow you to explore protein localization in situ. Also,you can use them in western blot analyses to confirm the proper fractionation of cell lysates.
Nucleus
Nucleus: a membrane-bound organelle found in eukaryotic cells.
We recommend
Anti-KDM1 / LSD1 antibody [EPR6825] - Nuclear Marker (ab129195)
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Nuclear pore
Nuclear pore: complexes that allow the selective exchange of cellular components, like RNA, proteins, and lipids, between the nucleus and the cytoplasm.
We recommend
Anti-NUP98 antibody [EPR6678] - Nuclear Pore Marker (ab124980)
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Nuclear envelope
Nuclear envelope: a membrane barrier that separates the nucleus from the cytoplasm. It comprises an inner and outer membrane with specialized nuclear pores and is separated by the perinuclear space.
We recommend
Anti-Lamin A + C antibody [EPR4100] - Nuclear Envelope Marker (ab108595)
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Nuclear speckles
Nuclear speckles: irregularly shaped structures of varied sizes, which are rich in splicing factors, including small nuclear ribonucleoprotein particles (snRNPs).
We recommend
Anti-SC35 antibody [SC-35] - Nuclear Speckle Marker (ab11826)
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Nucleolus
Nucleolus: found within the nucleus where it works as the site for ribosomal RNA (rRNA) gene transcription and the early steps of ribosome assembly.
We recommend
Anti-Fibrillarin antibody [EPR10823(B)] - Nucleolar Marker (ab166630)
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Heterochromatin
Heterochromatin: is densely packed chromatin within the nucleus inaccessible to DNA/RNA polymerases and is thus transcriptionally silent.
We recommend
Anti-HP1 alpha antibody [EPR5777] - Heterochromatin marker (ab109028)
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Centromere
Centromere: the region of linear chromosomes that join a pair of chromatids. Centromeres are where the mitotic spindle attaches.