Nuclear markers

Your guide to selecting nuclear markers.

Antibodies against specific organelles, the cell membrane, or cytoskeletal components allow you to explore protein localization  in situ.  Also,you can use them in western blot analyses to confirm the proper fractionation of cell lysates.

Nucleus

Nucleus:  a membrane-bound organelle found in eukaryotic cells.

We recommend

Anti-KDM1 / LSD1 antibody [EPR6825] - Nuclear Marker (ab129195)

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Nuclear pore

Nuclear pore:  complexes that allow the selective exchange of cellular components, like RNA, proteins, and lipids, between the nucleus and the cytoplasm.

We recommend

Anti-NUP98 antibody [EPR6678] - Nuclear Pore Marker (ab124980)

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Nuclear envelope

Nuclear envelope:   a membrane barrier that separates the nucleus from the cytoplasm. It comprises an inner and outer membrane with specialized nuclear pores and is separated by the perinuclear space.

We recommend

Anti-Lamin A + C antibody [EPR4100] - Nuclear Envelope Marker (ab108595)

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Nuclear speckles

Nuclear speckles: irregularly shaped structures of varied sizes, which are rich in splicing factors, including small nuclear ribonucleoprotein particles (snRNPs).

We recommend

Anti-SC35 antibody [SC-35] - Nuclear Speckle Marker (ab11826)

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Nucleolus

Nucleolus:  found within the nucleus where it works as the site for ribosomal RNA (rRNA) gene transcription and the early steps of ribosome assembly.

We recommend

Anti-Fibrillarin antibody [EPR10823(B)] - Nucleolar Marker (ab166630)

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Heterochromatin

Heterochromatin:  is densely packed chromatin within the nucleus inaccessible to DNA/RNA polymerases and is thus transcriptionally silent.

We recommend

Anti-HP1 alpha antibody [EPR5777] - Heterochromatin marker (ab109028)

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Centromere

Centromere: the region of linear chromosomes that join a pair of chromatids. Centromeres are where the mitotic spindle attaches.

We recommend

Anti-CENPA antibody [EP800Y] - Centromere Marker (ab45694)