Organelle markers

Your guide to selecting organelle markers.

Antibodies against specific organelles allow you to explore protein localization  in situ.  Also,you can use them in western blot analyses to confirm the proper fractionation of cell lysates.

Endoplasmic reticulum (ER)

Endoplasmic reticulum (ER): found in eukaryotic cells and is made of membrane sacs called cisternae. Rough ER (where ribosomes are bound) is a site of protein synthesis. Smooth ER is a site for lipid and carbohydrate metabolism. The ER forms part of a network of membranes with the Golgi and lysosomes.

We recommend

Anti-Calreticulin antibody [EPR3924] - ER Marker (ab92516)

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Golgi apparatus

Golgi apparatus:  serves as a molecular assembly line in which membrane proteins undergo extensive post-translational modification. The Golgi is part of a network of membranes with the ER and lysosomes.

We recommend

Anti-GM130 antibody [EP892Y] - cis-Golgi Marker (ab52649)

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Mitochondria

Mitochondria:  cytoplasmic organelles found in almost all eukaryotic cells, comprising an outer membrane, a folded inner membrane, and a matrix. Mitochondria are the cellular powerhouses, generating ATP through oxidative phosphorylation, and play a role in apoptosis.

We recommend

Anti-ATP5A antibody [15H4C4] - Mitochondrial Marker (ab14748)

Anti-TOMM20 antibody [EPR15581-39] - Mitochondrial Marker (ab186734)

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Ribosome

Ribosome:  an assembly of proteins and ribosomal RNAs (rRNAs) that translate messenger RNAs (mRNAs) into proteins.

We recommend

Anti-RPS3 antibody [EPR7808] - Ribosome Marker (ab128995)

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Lysosome

Lysosome:  membrane-bound organelle that breaks down macromolecules into constituent parts to then be recycled. Lysosomes form part of a network of membranes with the ER and Golgi.

We recommend

Anti-M6PR (cationindependent) antibody [EPR6599] - Lysosome Membrane Marker (ab124767)

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Endosome

Endosome:  formed at the plasma membrane or the Golgi, endosomes are membrane-delimited intracellular transporters that fuse with lysosomes, are involved in returning material to the plasma membrane.

We recommend

Anti-RAB7 antibody [EPR7588(B)] - Late Endosome Marker (ab126712)

Anti-EEA1 antibody [EPR4245] - Early Endosome Marker (ab109110)

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Peroxisome

Peroxisome:  cytoplasmic organelles responsible for catalyzing fatty acid via beta-oxidation and play a vital role in generating and degrading reactive oxygen species.

We recommend

Anti-Catalase antibody [EP1929Y] - Peroxisome Marker (ab76024)

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Autophagosome

Autophagosome: vesicles with a double membrane that contain cellular material destined for degradation via autophagy. The formation of autophagosomes depends on type III PI3K lipid kinase activity.

We recommend

Anti-SQSTM1 / p62 antibody [EPR4844] - Autophagosome Marker (ab109012)

Anti-LC3B antibody [EPR18709] - Autophagosome Marker (ab192890)

Autophagy marker antibody sampler panel (ab228525)

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