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High antibody elution in immunoprecipitation (IP)

Elution is done during the final step of IP.

High amount of antibody eluting

Possible causes


Too much antibody eluting with the target protein

  • Crosslinking the antibody to the beads before the immunoprecipitation and eluting using a gentle glycine buffer gradient should significantly reduce the amount of antibody eluted.

  • Try reducing the amount of antibody.