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High antibody elution in immunoprecipitation (IP)

Elution is done during the final step of IP.

High amount of antibody eluting

Possible causes

Solution

Too much antibody eluting with the target protein

  • Crosslinking the antibody to the beads before the immunoprecipitation and eluting using a gentle glycine buffer gradient should significantly reduce the amount of antibody eluted.

  • Try reducing the amount of antibody.