High antibody elution in immunoprecipitation (IP)

Elution is done during the final step of IP.

Why is a high amount of antibody eluting?

Possible causes

Solution

Too much antibody eluting with the target protein

Crosslinking the antibody to the beads before the immunoprecipitation and eluting using a gentle glycine buffer gradient should significantly reduce the amount of antibody eluted.
Try reducing the amount of antibody.