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High background

Find solutions to common problems for high background in ChIP

High background in non-specific antibody controls

Possible causes

Solution

Non-specific binding to Protein A or G beads

  • Include a pre-clearing step, where the lysed sample is mixed with beads alone for 1 hr and removed prior to adding the antibody.

Some Protein A or G beads give high background

  • Find a suitable supplier that provides the cleanest results with low background in the non-specific control.

The ChIP buffers may be contaminated

  • Prepare fresh lysis and wash solutions.