High event rate in Flow cytometry

Explore possible causes and solutions for high event rate in Flow cytometry.

Why is there a high event rate?

Possible causes

Solution

High number of cells.

Through overcaution or giving yourself too much room for error you may end up with a higher number of cells in your sample than needed. It's easy to dilute these to the necessary level.

Dilute to between 1x105 and 1x106 cells/ml.