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High side scatter background in Flow cytometry

Explore possible causes and solutions for high side scatter background from small particles in Flow cytometry.

How can I mitigate a high side scatter background (from small particles)?

Possible causes

Solution

Cells lysed.

Lysis can occur if samples are not fresh or prepared correctly.

  • Ensure cells in the sample have not lysed and broken up.

  • Do not centrifuge cells at a high rotor speed or vortex too violently.

Bacterial contamination

Bacteria auto fluoresce at a low level. This will also give a high event rate.

  • Ensure sample is not contaminated.Â