High side scatter background in Flow cytometry
Explore possible causes and solutions for high side scatter background from small particles in Flow cytometry.
How can I mitigate a high side scatter background (from small particles)?
Possible causes
Solution
Cells lysed.
Lysis can occur if samples are not fresh or prepared correctly.
-
Ensure cells in the sample have not lysed and broken up.
-
Do not centrifuge cells at a high rotor speed or vortex too violently.
Bacterial contamination
Bacteria auto fluoresce at a low level. This will also give a high event rate.
Ensure sample is not contaminated.