Low resolution with high background in ChIP
Explore causes of low resolution with high background in ChIP.
Why am I experiencing low resolution with high background across large regions?
Possible causes
Solution
DNA fragment size may be too large
DNA fragmentation should be optimized when using different cell types. Both sonication times and enzyme incubation times can vary.
- We suggest a DNA fragment size of no larger than 1.5 kbp. If chromatin is being digested using enzymes, mononucleosomes (175 bp) can be obtained.