Problems with PCR amplification in ChIP
Explore causes of problems with PCR amplification in ChIP.
Problems with PCR amplification on immunoprecipitated DNA
Possible causes
Solution
High signal in all samples after PCR, including no template control
- Real-time PCR solutions may be contaminated, we suggest preparing new solutions from stocks.
No DNA amplification in samples
- Be sure to include standard/input DNA to confirm that the primers are working well.