Skip to main content

Problems with PCR amplification in ChIP

Explore causes of problems with PCR amplification in ChIP.

Problems with PCR amplification on immunoprecipitated DNA

Possible causes


High signal in all samples after PCR, including no template control

  • Real-time PCR solutions may be contaminated, we suggest preparing new solutions from stocks.

No DNA amplification in samples

  • Be sure to include standard/input DNA to confirm that the primers are working well.