Weak or no western blotting signal
Explore solutions for weak or no western blotting signal
Why is there weak or no western blotting signal?
Possible causes
Solution
Film not exposed for long enough
- Expose the film for longer
Not enough antibody
- Increase the concentration of the antibody
Sample not given enough incubation time
- Extend incubation times
Not enough sample used
- Increase the sample amount
Inhibited HRP development
Using sodium azide in the secondary antibody solution inhibits HRP development.
- Try using antibodies in solutions with traditional carrier agents, like sodium azide, removed. Our carrier-free formulations are BSA, sodium azide, and glycerol-free.
Inhibited alkaline phosphatase blot development
Tween 20 may inhibit alkaline phosphatase blot development.
- Do not use Tween 20