Key features and details
- Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
- Rabbit monoclonal [EPR3282] to EN1 / Engrailed 1 - BSA and Azide free
- Suitable for: WB
- Reacts with: Mouse, Rat, Human
Product nameAnti-EN1 / Engrailed 1 antibody [EPR3282] - BSA and Azide free
See all EN1 / Engrailed 1 primary antibodies
DescriptionRabbit monoclonal [EPR3282] to EN1 / Engrailed 1 - BSA and Azide free
Tested applicationsSuitable for: WBmore details
Unsuitable for: ICC or IHC-P
Species reactivityReacts with: Mouse, Rat, Human
Synthetic peptide within Human EN1/ Engrailed 1. The exact sequence is proprietary.
Ab239934 is the carrier-free version of ab108598. This format is designed for use in antibody labeling, including fluorochromes, metal isotopes, oligonucleotides, enzymes.
Our carrier-free formats are supplied in a buffer free of BSA, sodium azide and glycerol for higher conjugation efficiency.
Use our conjugation kits for antibody conjugates that are ready-to-use in as little as 20 minutes with <1 minute hands-on-time and 100% antibody recovery: available for fluorescent dyes, HRP, biotin and gold.
ab239934 is compatible with the Maxpar® Antibody Labeling Kit from Fluidigm.
Maxpar® is a trademark of Fluidigm Canada Inc.
This product is a recombinant monoclonal antibody, which offers several advantages including:
- - High batch-to-batch consistency and reproducibility
- - Improved sensitivity and specificity
- - Long-term security of supply
- - Animal-free production
Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.
Storage instructionsShipped at 4°C. Store at +4°C. Do Not Freeze.
Storage bufferConstituent: PBS
Concentration information loading...
PurityProtein A purified
Our Abpromise guarantee covers the use of ab239934 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
|WB||Use at an assay dependent concentration. Detects a band of approximately 55 kDa (predicted molecular weight: 40 kDa).|
Sequence similaritiesBelongs to the engrailed homeobox family.
Contains 1 homeobox DNA-binding domain.
- Information by UniProt
- En 1 antibody
- EN1 antibody
- Engrailed 1 antibody
All lanes : Anti-EN1 / Engrailed 1 antibody [EPR3282] (ab108598) at 1/1000 dilution
Lane 1 : U87-MG lysate
Lane 2 : SH-SY5Y lysate
Lane 3 : 293T lysate
Lane 4 : Human fetal brain lysate
Lysates/proteins at 10 µg per lane.
Predicted band size: 40 kDa
This data was developed using ab108598, the same antibody clone in a different buffer formulation.
To our knowledge, customised protocols are not required for this product. Please try the standard protocols listed below and let us know how you get on.
ab239934 has not yet been referenced specifically in any publications.