Product nameAnti-EN1 / Engrailed 1 antibody
See all EN1 / Engrailed 1 primary antibodies
DescriptionRabbit polyclonal to EN1 / Engrailed 1
Tested applicationsSuitable for: WB, ELISAmore details
Species reactivityReacts with: Human
Predicted to work with: Mouse, Chicken, Zebrafish
- HepG2 whole cell lysate (ab7900).
Storage instructionsShipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid repeated freeze / thaw cycles.
Storage bufferPreservative: 0.09% Sodium azide
Constituents: 2% Sucrose, PBS
Concentration information loading...
PurityImmunogen affinity purified
Purification notesab83693 is purified by a peptide affinity chromatography method.
Our Abpromise guarantee covers the use of ab83693 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
|WB||Use a concentration of 1 µg/ml. Predicted molecular weight: 40 kDa. Good results were obtained when blocked with 5% non-fat dry milk in 0.05% PBS-T.|
|ELISA||Use at an assay dependent concentration.
Titre using peptide based assay: 1/312500.
Sequence similaritiesBelongs to the engrailed homeobox family.
Contains 1 homeobox DNA-binding domain.
- Information by UniProt
- En 1 antibody
- EN1 antibody
- Engrailed 1 antibody
Anti-EN1 / Engrailed 1 antibody (ab83693) at 1 µg/ml + HepG2 cell lysate at 10 µg
HRP conjugated anti-Rabbit IgG at 1/50000 dilution
Predicted band size: 40 kDa
Observed band size: 37 kDa why is the actual band size different from the predicted?
Additional bands at: 24 kDa. We are unsure as to the identity of these extra bands.
This product has been referenced in:
- Kim YJ et al. Engrailed 1 overexpression as a potential prognostic marker in quintuple-negative breast cancer. Cancer Biol Ther 19:335-345 (2018). Read more (PubMed: 29333926) »
- Beltran AS et al. Novel role of Engrailed 1 as a prosurvival transcription factor in basal-like breast cancer and engineering of interference peptides block its oncogenic function. Oncogene N/A:N/A (2013). Read more (PubMed: 24141779) »