Key features and details
- Rabbit polyclonal to EN2
- Suitable for: IP, WB
- Reacts with: Human
- Isotype: IgG
Product nameAnti-EN2 antibody
See all EN2 primary antibodies
DescriptionRabbit polyclonal to EN2
Tested applicationsSuitable for: IP, WBmore details
Species reactivityReacts with: Human
Recombinant full length protein corresponding to Human EN2.
Database link: P19622
- WB: MCF7 whole cell lysate. IP: HeLa whole cell lysate.
Reproducibility is key to advancing scientific discovery and accelerating scientists’ next breakthrough.
Abcam is leading the way with our range of recombinant antibodies, knockout-validated antibodies and knockout cell lines, all of which support improved reproducibility.
We are also planning to innovate the way in which we present recommended applications and species on our product datasheets, so that only applications & species that have been tested in our own labs, our suppliers or by selected trusted collaborators are covered by our Abpromise™ guarantee.
In preparation for this, we have started to update the applications & species that this product is Abpromise guaranteed for.
We are also updating the applications & species that this product has been “predicted to work with,” however this information is not covered by our Abpromise guarantee.
Applications & species from publications and Abreviews that have not been tested in our own labs or in those of our suppliers are not covered by the Abpromise guarantee.
Please check that this product meets your needs before purchasing. If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, as well as customer reviews and Q&As.
Storage instructionsShipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle.
Storage bufferpH: 7.00
Preservative: 0.01% Thimerosal (merthiolate)
Constituents: 78.99% PBS, 1% BSA, 20% Glycerol (glycerin, glycerine)
Concentration information loading...
PurityImmunogen affinity purified
Our Abpromise guarantee covers the use of ab227167 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
|IP||1/100 - 1/500.|
|WB||1/5000 - 1/20000. Predicted molecular weight: 34 kDa.|
RelevanceEN1 and EN2 encode homeodomain containing proteins and have been implicated in the control of pattern formation during development of the central nervous system. EN2 may play a role in susceptibility to autism spectrum disorders.
- AUTS 1 antibody
- AUTS1 antibody
- EN 2 antibody
Anti-EN2 antibody (ab227167) at 1/10000 dilution + MCF7 (human breast adenocarcinoma cell line) whole cell lysate at 30 µg
Predicted band size: 34 kDa
12% SDS-PAGE gel.
EN2 was immunoprecipitated from HeLa (human epithelial cell line from cervix adenocarcinoma) whole cell lysate with 2 µg of ab227167. Western blot was performed from the immunoprecipitate using ab227167 at 1/1000 dilution. Anti-Rabbit IgG was used as secondary antibody.
Lane 1: HeLa whole cell lysate 40 µg (Input).
Lane 2: 2 µg preimmune rabbit IgG instead of ab227167 in HeLa whole cell lysate.
Lane 3: ab227167 IP in HeLa whole cell lysate.
To our knowledge, customised protocols are not required for this product. Please try the standard protocols listed below and let us know how you get on.
ab227167 has not yet been referenced specifically in any publications.