Overview

  • Product name

  • Description

    Rabbit polyclonal to EN2
  • Host species

    Rabbit
  • Tested applications

    Suitable for: IP, WBmore details
  • Species reactivity

    Reacts with: Human
  • Immunogen

    Recombinant full length protein corresponding to Human EN2.
    Database link: P19622

  • Positive control

    • WB: MCF7 whole cell lysate. IP: HeLa whole cell lysate.

Properties

Applications

Our Abpromise guarantee covers the use of ab227167 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
IP 1/100 - 1/500.
WB 1/5000 - 1/20000. Predicted molecular weight: 34 kDa.

Target

  • Relevance

    EN1 and EN2 encode homeodomain containing proteins and have been implicated in the control of pattern formation during development of the central nervous system. EN2 may play a role in susceptibility to autism spectrum disorders.
  • Cellular localization

    Nuclear
  • Database links

  • Alternative names

    • AUTS 1 antibody
    • AUTS1 antibody
    • EN 2 antibody
    • Engrailed 2 antibody
    • Engrailed homeo box 2 antibody
    • Engrailed homeobox 2 antibody
    • Engrailed homolog 2 antibody
    • Engrailed2 antibody
    • Homeobox protein engrailed 2 antibody
    • Homeobox protein engrailed2 antibody
    • Hu En 2 antibody
    • HuEn2 antibody
    • MabEn antibody
    see all

Images

  • Anti-EN2 antibody (ab227167) at 1/10000 dilution + MCF7 (human breast adenocarcinoma cell line) whole cell lysate at 30 µg

    Predicted band size: 34 kDa



    12% SDS-PAGE gel.

  • EN2 was immunoprecipitated from HeLa (human epithelial cell line from cervix adenocarcinoma) whole cell lysate with 2 µg of ab227167. Western blot was performed from the immunoprecipitate using ab227167 at 1/1000 dilution. Anti-Rabbit IgG was used as secondary antibody.

    Lane 1: HeLa whole cell lysate 40 µg (Input). 

    Lane 2: 2 µg preimmune rabbit IgG instead of ab227167 in HeLa whole cell lysate.

    Lane 3: ab227167 IP in HeLa whole cell lysate. 

    12% SDS-PAGE.

     

References

ab227167 has not yet been referenced specifically in any publications.

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