Overview

  • Product name

  • Description

    Goat polyclonal to EN2
  • Host species

    Goat
  • Tested applications

    Suitable for: WBmore details
  • Species reactivity

    Reacts with: Mouse
    Predicted to work with: Rat, Chicken, Dog, Human
  • Immunogen

    Synthetic peptide:

    C-NHSTTAKEGKSDSE

    , corresponding to C terminal amino acids 320-333 of Human EN2

  • Positive control

    • Mouse and rat brain lysates.

Properties

Applications

Our Abpromise guarantee covers the use of ab45867 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB Use a concentration of 0.1 - 0.3 µg/ml. Detects a band of approximately 30 kDa (predicted molecular weight: 34 kDa). 34kDa band observed in rat brain lysate.

Target

  • Relevance

    EN1 and EN2 encode homeodomain containing proteins and have been implicated in the control of pattern formation during development of the central nervous system. EN2 may play a role in susceptibility to autism spectrum disorders.
  • Cellular localization

    Nuclear
  • Database links

  • Alternative names

    • AUTS 1 antibody
    • AUTS1 antibody
    • EN 2 antibody
    • Engrailed 2 antibody
    • Engrailed homeo box 2 antibody
    • Engrailed homeobox 2 antibody
    • Engrailed homolog 2 antibody
    • Engrailed2 antibody
    • Homeobox protein engrailed 2 antibody
    • Homeobox protein engrailed2 antibody
    • Hu En 2 antibody
    • HuEn2 antibody
    • MabEn antibody
    see all

Images

  • Anti-EN2 antibody (ab45867) at 1 µg/ml + Mouse brain lysate at 35 µg

    Predicted band size: 34 kDa

  • Immunofluorescence analysis of paraformaldehyde fixed HEK293 cells, permeabilized with 0.15% Triton. Incubated with ab45867 for 1hr (10 μg/ml) followed by Alexa Fluor 488 secondary antibody (2 μg/ml), showing nuclear staining. Actin filaments were stained with phalloidin (red) and the nuclear stain is DAPI (blue). Negative control: Unimmunized goat IgG (10ug/ml) followed by Alexa Fluor 488 secondary antibody (2 μg/ml).

References

This product has been referenced in:

  • Zhang X  et al. Retinal defects in mice lacking the autism-associated gene Engrailed-2. Neuroscience 408:177-190 (2019). Read more (PubMed: 30980901) »
  • Provenzano G  et al. Hippocampal dysregulation of neurofibromin-dependent pathways is associated with impaired spatial learning in engrailed 2 knock-out mice. J Neurosci 34:13281-8 (2014). Mouse . Read more (PubMed: 25274808) »
See all 4 Publications for this product

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