Key features and details
- Rabbit polyclonal to ENAH/MENA
- Suitable for: WB, IP
- Reacts with: Human
- Isotype: IgG
Product nameAnti-ENAH/MENA antibody
See all ENAH/MENA primary antibodies
DescriptionRabbit polyclonal to ENAH/MENA
Tested applicationsSuitable for: WB, IPmore details
Species reactivityReacts with: Human
Predicted to work with: Mouse, Rat, Horse, Chicken, Guinea pig, Cow, Turkey, Chimpanzee, Cynomolgus monkey, Rhesus monkey, Gorilla, Orangutan
- HeLa whole cell lysate (ab150035).
This product was previously labelled as ENAH
Storage instructionsShipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle.
Storage bufferPreservative: 0.09% Sodium azide
Constituents: 99% Tris buffered saline, 0.1% BSA
Concentration information loading...
PurityImmunogen affinity purified
Purification notesab176820 was affinity purified using an epitope specific to ENAH/MENA immobilized on solid support.
Our Abpromise guarantee covers the use of ab176820 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
|WB||1/2000 - 1/10000. Predicted molecular weight: 67 kDa.|
|IP||Use at 2-5 µg/mg of lysate.|
FunctionEna/VASP proteins are actin-associated proteins involved in a range of processes dependent on cytoskeleton remodeling and cell polarity such as axon guidance and lamellipodial and filopodial dynamics in migrating cells. ENAH induces the formation of F-actin rich outgrowths in fibroblasts. Acts synergistically with BAIAP2-alpha and downstream of NTN1 to promote filipodia formation. Required for the actin-based mobility of Listeria monocytogenes.
Tissue specificityExpressed in myoepithelia of parotid, breast, bronchial glands and sweat glands. Expressed in colon-rectum muscolaris mucosae epithelium, pancreas acinar ductal epithelium, endometrium epithelium, prostate fibromuscolar stroma and placenta vascular media. Overexpressed in a majority of breast cancer cell lines and primary breast tumor lesions.
Sequence similaritiesBelongs to the Ena/VASP family.
Contains 1 WH1 domain.
DomainThe EVH2 domain is comprised of 3 regions. Block A is a thymosin-like domain required for G-actin binding. The KLKR motif within this block is essential for the G-actin binding and for actin polymerization. Block B is required for F-actin binding and subcellular location, and Block C for tetramerization.
modificationsNTN1-induced PKA phosphorylation on Ser-265 directly parallels the formation of filopodial protrusions.
Phosphorylated upon DNA damage, probably by ATM or ATR.
Cellular localizationCytoplasm. Cytoplasm > cytoskeleton. Cell projection > lamellipodium. Cell projection > filopodium. Cell junction > synapse. Cell junction > focal adhesion. Targeted to the leading edge of lamellipodia and filopodia by MRL family members. Colocalizes at filopodial tips with a number of other proteins including vinculin and zyxlin. Colocalizes with N-WASP at the leading edge. Colocalizes with GPHN and PFN at synapses.
- Information by UniProt
- ENA antibody
- Enah antibody
- ENAH_HUMAN antibody
All lanes : Anti-ENAH/MENA antibody (ab176820) at 0.04 µg/ml
Lane 1 : HeLa whole cell lysate at 50 µg
Lane 2 : HeLa whole cell lysate at 15 µg
Lane 3 : HeLa whole cell lysate at 5 µg
Developed using the ECL technique.
Predicted band size: 67 kDa
Exposure time: 10 seconds
Detection of ENAH/MENA in Immunoprecipitates of HeLa whole cell lysate (1 mg for IP, 20% of IP loaded) using ab176820 at 3 µg/mg lysate for IP (Lane 1). For WB detection, ab176920 was used at 0.1 µg/ml. Lane 2 represents control IgG IP. Detection: Chemiluminescence with an exposure time of 10 seconds.
ab176820 has not yet been referenced specifically in any publications.