• Product name

  • Description

    Rabbit polyclonal to ENAH/MENA
  • Host species

  • Tested applications

    Suitable for: ICC/IF, WB, IHC-Pmore details
  • Species reactivity

    Reacts with: Human
  • Immunogen

    Recombinant fragment corresponding to Human ENAH/MENA aa 486-589.


    Database link: Q8N8S7

  • Positive control

    • IHC-P: Human prostate, cerebral cortex and testis tissues. WB: U-2 OS and MCF7 cell lysates. ICC/IF: U-2 OS cells.
  • General notes

     This product was previously labelled as ENAH




Our Abpromise guarantee covers the use of ab244417 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
ICC/IF Use a concentration of 1 - 4 µg/ml.

Fixation/Permeabilization: PFA/Triton X-100.

WB Use a concentration of 0.4 µg/ml. Predicted molecular weight: 67 kDa.
IHC-P 1/200 - 1/500. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.


  • Function

    Ena/VASP proteins are actin-associated proteins involved in a range of processes dependent on cytoskeleton remodeling and cell polarity such as axon guidance and lamellipodial and filopodial dynamics in migrating cells. ENAH induces the formation of F-actin rich outgrowths in fibroblasts. Acts synergistically with BAIAP2-alpha and downstream of NTN1 to promote filipodia formation. Required for the actin-based mobility of Listeria monocytogenes.
  • Tissue specificity

    Expressed in myoepithelia of parotid, breast, bronchial glands and sweat glands. Expressed in colon-rectum muscolaris mucosae epithelium, pancreas acinar ductal epithelium, endometrium epithelium, prostate fibromuscolar stroma and placenta vascular media. Overexpressed in a majority of breast cancer cell lines and primary breast tumor lesions.
  • Sequence similarities

    Belongs to the Ena/VASP family.
    Contains 1 WH1 domain.
  • Domain

    The EVH2 domain is comprised of 3 regions. Block A is a thymosin-like domain required for G-actin binding. The KLKR motif within this block is essential for the G-actin binding and for actin polymerization. Block B is required for F-actin binding and subcellular location, and Block C for tetramerization.
  • Post-translational

    NTN1-induced PKA phosphorylation on Ser-265 directly parallels the formation of filopodial protrusions.
    Phosphorylated upon DNA damage, probably by ATM or ATR.
  • Cellular localization

    Cytoplasm. Cytoplasm > cytoskeleton. Cell projection > lamellipodium. Cell projection > filopodium. Cell junction > synapse. Cell junction > focal adhesion. Targeted to the leading edge of lamellipodia and filopodia by MRL family members. Colocalizes at filopodial tips with a number of other proteins including vinculin and zyxlin. Colocalizes with N-WASP at the leading edge. Colocalizes with GPHN and PFN at synapses.
  • Information by UniProt
  • Database links

  • Alternative names

    • ENA antibody
    • Enah antibody
    • ENAH_HUMAN antibody
    • MENA antibody
    • NDPP1 antibody
    • Protein enabled homolog antibody
    see all


  • Paraffin-embedded human testis tissue stained for ENAH/MENA using ab244417 at 1/200 dilution in immunohistochemical analysis.

  • All lanes : Anti-ENAH/MENA antibody (ab244417) at 0.4 µl

    Lane 1 : U-2 OS (human bone osteosarcoma epithelial cell line) cell lysate
    Lane 2 : MCF7 (human breast adenocarcinoma cell line) cell lysate

    Predicted band size: 67 kDa

  • Paraffin-embedded human cerebral cortex tissue stained for ENAH/MENA using ab244417 at 1/200 dilution in immunohistochemical analysis.

  • Paraffin-embedded human prostate tissue stained for ENAH/MENA using ab244417 at 1/200 dilution in immunohistochemical analysis.

  • Paraffin-embedded human liver tissue stained for ENAH/MENA using ab244417 at 1/200 dilution in immunohistochemical analysis.

  • PFA-fixed, Triton X-100 permeabilized U-2 OS (human bone osteosarcoma epithelial cell line) cells stained for ENAH/MENA (green) using ab244417 at 4 µg/ml in ICC/IF.


ab244417 has not yet been referenced specifically in any publications.

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