Overview

  • Product name

  • Description

    Rabbit polyclonal to ENAH/MENA
  • Host species

    Rabbit
  • Tested applications

    Suitable for: IHC-P, WB, ICC/IFmore details
  • Species reactivity

    Reacts with: Mouse, Rat, Human
  • Immunogen

    Recombinant fragment corresponding to Human ENAH/MENA aa 226-316.
    Sequence:

    RQERQERERLERLERERQERERQEQLEREQLEWERERRISSAAAPASVET PLNSVLGDSSASEPGLQAASQPAETPSQQGIVLGPLAPPPP


    Database link: Q8N8S7

  • Positive control

    • IHC-P: Human prostate, cerebral cortex and testis tissues. WB: U-2 OS, MCF7, NIH/3T3 and NBT-II cell lysates. ICC/IF: U-2 OS cells.
  • General notes

     This product was previously labelled as ENAH

     

Properties

Applications

Our Abpromise guarantee covers the use of ab244423 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
IHC-P 1/200 - 1/500. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.
WB Use a concentration of 0.4 µg/ml. Predicted molecular weight: 67 kDa.
ICC/IF Use a concentration of 1 - 4 µg/ml.

Fixation/Permeabilization: PFA/Triton X-100.

Target

  • Function

    Ena/VASP proteins are actin-associated proteins involved in a range of processes dependent on cytoskeleton remodeling and cell polarity such as axon guidance and lamellipodial and filopodial dynamics in migrating cells. ENAH induces the formation of F-actin rich outgrowths in fibroblasts. Acts synergistically with BAIAP2-alpha and downstream of NTN1 to promote filipodia formation. Required for the actin-based mobility of Listeria monocytogenes.
  • Tissue specificity

    Expressed in myoepithelia of parotid, breast, bronchial glands and sweat glands. Expressed in colon-rectum muscolaris mucosae epithelium, pancreas acinar ductal epithelium, endometrium epithelium, prostate fibromuscolar stroma and placenta vascular media. Overexpressed in a majority of breast cancer cell lines and primary breast tumor lesions.
  • Sequence similarities

    Belongs to the Ena/VASP family.
    Contains 1 WH1 domain.
  • Domain

    The EVH2 domain is comprised of 3 regions. Block A is a thymosin-like domain required for G-actin binding. The KLKR motif within this block is essential for the G-actin binding and for actin polymerization. Block B is required for F-actin binding and subcellular location, and Block C for tetramerization.
  • Post-translational
    modifications

    NTN1-induced PKA phosphorylation on Ser-265 directly parallels the formation of filopodial protrusions.
    Phosphorylated upon DNA damage, probably by ATM or ATR.
  • Cellular localization

    Cytoplasm. Cytoplasm > cytoskeleton. Cell projection > lamellipodium. Cell projection > filopodium. Cell junction > synapse. Cell junction > focal adhesion. Targeted to the leading edge of lamellipodia and filopodia by MRL family members. Colocalizes at filopodial tips with a number of other proteins including vinculin and zyxlin. Colocalizes with N-WASP at the leading edge. Colocalizes with GPHN and PFN at synapses.
  • Information by UniProt
  • Database links

  • Alternative names

    • ENA antibody
    • Enah antibody
    • ENAH_HUMAN antibody
    • MENA antibody
    • NDPP1 antibody
    • Protein enabled homolog antibody
    see all

Images

  • Paraffin-embedded human testis tissue stained for ENAH/MENA using ab244423 at 1/200 dilution in immunohistochemical analysis.

  • All lanes : Anti-ENAH/MENA antibody (ab244423) at 0.4 µg/ml

    Lane 1 : NIH/3T3 (mouse embyro fibroblast cell line) cell lysate
    Lane 2 : NBT-II cell lysate

    Predicted band size: 67 kDa

  • All lanes : Anti-ENAH/MENA antibody (ab244423) at 0.4 µg/ml

    Lane 1 : U-2 OS (human bone osteosarcoma epithelial cell line) cell lysate
    Lane 2 : MCF7 (human breast adenocarcinoma cell line) cell lysate

    Predicted band size: 67 kDa

  • Paraffin-embedded human cerebral cortex tissue stained for ENAH/MENA using ab244423 at 1/200 dilution in immunohistochemical analysis.

  • Paraffin-embedded human liver tissue stained for ENAH/MENA using ab244423 at 1/200 dilution in immunohistochemical analysis.

  • Paraffin-embedded human prostate tissue stained for ENAH/MENA using ab244423 at 1/200 dilution in immunohistochemical analysis.

  • PFA-fixed, Triton X-100 permeabilized U-2 OS (human bone osteosarcoma epithelial cell line) cells stained for ENAH/MENA (green) using ab244423 at 4 µg/ml in ICC/IF.

References

ab244423 has not yet been referenced specifically in any publications.

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