Product nameAnti-ENAH/MENA antibody [EPR5660]
See all ENAH/MENA primary antibodies
DescriptionRabbit monoclonal [EPR5660] to ENAH/MENA
Tested applicationsSuitable for: WB, IHC-P, Flow Cytmore details
Unsuitable for: ICC or IP
Species reactivityReacts with: Mouse, Human
Does not react with: Rat
corresponding to Human ENAH/MENA aa 550-650.
Database link: Q8N8S7
- MDA-MB-435, MCF7, HUVEC and A431 cell lysates; Human Uterus tissue.
This product was previously labelled as ENAH
This product is a recombinant monoclonal antibody, which offers several advantages including:
- - High batch-to-batch consistency and reproducibility
- - Improved sensitivity and specificity
- - Long-term security of supply
- - Animal-free production
Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.
Storage instructionsShipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid freeze / thaw cycles.
Storage bufferpH: 7.20
Preservative: 0.05% Sodium azide
Constituents: 40% Glycerol, 9.85% Tris glycine, 50% Tissue culture supernatant
Concentration information loading...
PurityTissue culture supernatant
Our Abpromise guarantee covers the use of ab124685 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
|WB||1/1000 - 1/10000. Detects a band of approximately 80 kDa (predicted molecular weight: 67 kDa).|
|IHC-P||1/500 - 1/1000. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.
Antigen rerieval is recommended.
|Flow Cyt||1/100 - 1/500.
ab172730 - Rabbit monoclonal IgG, is suitable for use as an isotype control with this antibody.
FunctionEna/VASP proteins are actin-associated proteins involved in a range of processes dependent on cytoskeleton remodeling and cell polarity such as axon guidance and lamellipodial and filopodial dynamics in migrating cells. ENAH induces the formation of F-actin rich outgrowths in fibroblasts. Acts synergistically with BAIAP2-alpha and downstream of NTN1 to promote filipodia formation. Required for the actin-based mobility of Listeria monocytogenes.
Tissue specificityExpressed in myoepithelia of parotid, breast, bronchial glands and sweat glands. Expressed in colon-rectum muscolaris mucosae epithelium, pancreas acinar ductal epithelium, endometrium epithelium, prostate fibromuscolar stroma and placenta vascular media. Overexpressed in a majority of breast cancer cell lines and primary breast tumor lesions.
Sequence similaritiesBelongs to the Ena/VASP family.
Contains 1 WH1 domain.
DomainThe EVH2 domain is comprised of 3 regions. Block A is a thymosin-like domain required for G-actin binding. The KLKR motif within this block is essential for the G-actin binding and for actin polymerization. Block B is required for F-actin binding and subcellular location, and Block C for tetramerization.
modificationsNTN1-induced PKA phosphorylation on Ser-265 directly parallels the formation of filopodial protrusions.
Phosphorylated upon DNA damage, probably by ATM or ATR.
Cellular localizationCytoplasm. Cytoplasm > cytoskeleton. Cell projection > lamellipodium. Cell projection > filopodium. Cell junction > synapse. Cell junction > focal adhesion. Targeted to the leading edge of lamellipodia and filopodia by MRL family members. Colocalizes at filopodial tips with a number of other proteins including vinculin and zyxlin. Colocalizes with N-WASP at the leading edge. Colocalizes with GPHN and PFN at synapses.
- Information by UniProt
- ENA antibody
- Enah antibody
- ENAH_HUMAN antibody
ab124685, at 1/500 dilution, staining ENAH/MENA in formalin-fixed, paraffin-embedded Human uterus tissue, by Immunohistochemistry.
Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.
All lanes : Anti-ENAH/MENA antibody [EPR5660] (ab124685) at 1/1000 dilution
Lane 1 : MDA-MB-435 cell lysate
Lane 2 : MCF7 cell lysate
Lane 3 : HUVEC cell lysate
Lane 4 : A431 cell lysate
Lysates/proteins at 10 µg per lane.
All lanes : Goat anti-rabbit HRP at 1/2000 dilution
Predicted band size: 67 kDa
Observed band size: 80 kDa why is the actual band size different from the predicted?
This product has been referenced in:
- Li CF et al. Characterization of gene amplification-driven SKP2 overexpression in myxofibrosarcoma: potential implications in tumor progression and therapeutics. Clin Cancer Res 18:1598-610 (2012). Read more (PubMed: 22322669) »