RecombinantRabMAb

Recombinant Anti-ENAH/MENA antibody [EPR5660] (ab124685)

Submit a review Submit a question References (2)
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-ENAH/MENA antibody [EPR5660] (ab124685)
  • Western blot - Anti-ENAH/MENA antibody [EPR5660] (ab124685)
  • Anti-ENAH/MENA antibody [EPR5660] (ab124685)

Key features and details

  • Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
  • Rabbit monoclonal [EPR5660] to ENAH/MENA
  • Suitable for: WB, IHC-P
  • Reacts with: Human

Overview

  • Product name

    Anti-ENAH/MENA antibody [EPR5660]
    See all ENAH/MENA primary antibodies

  • Description

    Rabbit monoclonal [EPR5660] to ENAH/MENA

  • Host species

    Rabbit

  • Tested applications

    Suitable for: WB, IHC-Pmore details
    Unsuitable for: ICC or IP

  • Species reactivity

    Reacts with: Human
    Predicted to work with: MouseDoes not react with: Rat

  • Immunogen

    corresponding to Human ENAH/MENA aa 550-650.
    Database link: Q8N8S7

  • Positive control

    • MDA-MB-435, MCF7, HUVEC and A431 cell lysates; Human Uterus tissue.

  • General notes

     

     

     This product was previously labelled as ENAH

     

    This product is a recombinant monoclonal antibody, which offers several advantages including:

    • - High batch-to-batch consistency and reproducibility
    • - Improved sensitivity and specificity
    • - Long-term security of supply
    • - Animal-free production
    For more information see here.

    Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.

Properties

Applications

The Abpromise guarantee

Our Abpromise guarantee covers the use of ab124685 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB
1/1000 - 1/10000. Detects a band of approximately 80 kDa (predicted molecular weight: 67 kDa).
IHC-P
1/500 - 1/1000. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.

Antigen rerieval is recommended.
Notes
WB
1/1000 - 1/10000. Detects a band of approximately 80 kDa (predicted molecular weight: 67 kDa).
IHC-P
1/500 - 1/1000. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.

Antigen rerieval is recommended.
  • Application notes
    Is unsuitable for ICC or IP.

    • Target

    • Function

      Ena/VASP proteins are actin-associated proteins involved in a range of processes dependent on cytoskeleton remodeling and cell polarity such as axon guidance and lamellipodial and filopodial dynamics in migrating cells. ENAH induces the formation of F-actin rich outgrowths in fibroblasts. Acts synergistically with BAIAP2-alpha and downstream of NTN1 to promote filipodia formation. Required for the actin-based mobility of Listeria monocytogenes.

    • Tissue specificity

      Expressed in myoepithelia of parotid, breast, bronchial glands and sweat glands. Expressed in colon-rectum muscolaris mucosae epithelium, pancreas acinar ductal epithelium, endometrium epithelium, prostate fibromuscolar stroma and placenta vascular media. Overexpressed in a majority of breast cancer cell lines and primary breast tumor lesions.

    • Sequence similarities

      Belongs to the Ena/VASP family.
      Contains 1 WH1 domain.

    • Domain

      The EVH2 domain is comprised of 3 regions. Block A is a thymosin-like domain required for G-actin binding. The KLKR motif within this block is essential for the G-actin binding and for actin polymerization. Block B is required for F-actin binding and subcellular location, and Block C for tetramerization.

    • Post-translational
      modifications

      NTN1-induced PKA phosphorylation on Ser-265 directly parallels the formation of filopodial protrusions.
      Phosphorylated upon DNA damage, probably by ATM or ATR.

    • Cellular localization

      Cytoplasm. Cytoplasm > cytoskeleton. Cell projection > lamellipodium. Cell projection > filopodium. Cell junction > synapse. Cell junction > focal adhesion. Targeted to the leading edge of lamellipodia and filopodia by MRL family members. Colocalizes at filopodial tips with a number of other proteins including vinculin and zyxlin. Colocalizes with N-WASP at the leading edge. Colocalizes with GPHN and PFN at synapses.
    • Information by UniProt

    • Database links

      see all

    • Alternative names

      • ENA antibody
      • Enah antibody
      • ENAH_HUMAN antibody
      • MENA antibody
      • NDPP1 antibody
      • Protein enabled homolog antibody
      see all

    Images

    • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-ENAH/MENA antibody [EPR5660] (ab124685)
      Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-ENAH/MENA antibody [EPR5660] (ab124685)

      ab124685, at 1/500 dilution, staining ENAH/MENA in formalin-fixed, paraffin-embedded Human uterus tissue, by Immunohistochemistry.

      Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.

    • Western blot - Anti-ENAH/MENA antibody [EPR5660] (ab124685)
      Western blot - Anti-ENAH/MENA antibody [EPR5660] (ab124685)
      All lanes : Anti-ENAH/MENA antibody [EPR5660] (ab124685) at 1/1000 dilution

      Lane 1 : MDA-MB-435 (Human ductal carcinoma cell line) cell lysate
      Lane 2 : MCF7 cell lysate
      Lane 3 : HUVEC cell lysate
      Lane 4 : A431 cell lysate

      Lysates/proteins at 10 µg per lane.

      Secondary
      All lanes : Goat anti-rabbit HRP at 1/2000 dilution

      Predicted band size: 67 kDa
      Observed band size: 80 kDa why is the actual band size different from the predicted?

    • Anti-ENAH/MENA antibody [EPR5660] (ab124685)
      Anti-ENAH/MENA antibody [EPR5660] (ab124685)

    References (2)

    Publishing research using ab124685? Please let us know so that we can cite the reference in this datasheet.

    ab124685 has been referenced in 2 publications.

    • He Z  et al. Human umbilical cord mesenchymal stem cells-derived exosomes deliver microRNA-375 to downregulate ENAH and thus retard esophageal squamous cell carcinoma progression. J Exp Clin Cancer Res 39:140 (2020). PubMed: 32698859
    • Li CF  et al. Characterization of gene amplification-driven SKP2 overexpression in myxofibrosarcoma: potential implications in tumor progression and therapeutics. Clin Cancer Res 18:1598-610 (2012). PubMed: 22322669

    Customer reviews and Q&As

    Show All Reviews Q&A
    Submit a review Submit a question

    There are currently no Customer reviews or Questions for ab124685.
    Please use the links above to contact us or submit feedback about this product.

    Please note: All products are "FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC PROCEDURES"
    For licensing inquiries, please contact partnerships@abcam.com