Recombinant Anti-ENC1 antibody [EPR5406(B)] (ab124902)
Key features and details
- Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
- Rabbit monoclonal [EPR5406(B)] to ENC1
- Suitable for: WB, IHC-P
- Reacts with: Human
Related conjugates and formulations
Overview
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Product name
Anti-ENC1 antibody [EPR5406(B)]
See all ENC1 primary antibodies -
Description
Rabbit monoclonal [EPR5406(B)] to ENC1 -
Host species
Rabbit -
Tested applications
Suitable for: WB, IHC-Pmore details
Unsuitable for: IP -
Species reactivity
Reacts with: Human
Predicted to work with: Mouse, Rat -
Immunogen
Synthetic peptide within Human ENC1 aa 550-650. The exact sequence is proprietary.
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Positive control
- Human fetal brain lysate; Human brain tissue
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General notes
This product is a recombinant monoclonal antibody, which offers several advantages including:
- - High batch-to-batch consistency and reproducibility
- - Improved sensitivity and specificity
- - Long-term security of supply
- - Animal-free production
Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.
Properties
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Form
Liquid -
Storage instructions
Shipped at 4°C. Store at -20°C. Stable for 12 months at -20°C. -
Dissociation constant (KD)
KD = 1.03 x 10 -10 M Learn more about KD -
Storage buffer
pH: 7.20
Preservative: 0.05% Sodium azide
Constituents: 40% Glycerol (glycerin, glycerine), 9.85% Tris glycine, 50% Tissue culture supernatant -
Concentration information loading...
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Purity
Tissue culture supernatant -
Clonality
Monoclonal -
Clone number
EPR5406(B) -
Isotype
IgG -
Research areas
Associated products
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Alternative Versions
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Isotype control
Applications
The Abpromise guarantee
Our Abpromise guarantee covers the use of ab124902 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
Application | Abreviews | Notes |
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WB |
1/1000 - 1/10000. Detects a band of approximately 66 kDa (predicted molecular weight: 66 kDa).
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IHC-P |
1/100 - 1/250. Perform heat mediated antigen retrieval before commencing with IHC staining protocol.
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Notes |
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WB
1/1000 - 1/10000. Detects a band of approximately 66 kDa (predicted molecular weight: 66 kDa). |
IHC-P
1/100 - 1/250. Perform heat mediated antigen retrieval before commencing with IHC staining protocol. |
Target
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Function
Actin-binding protein involved in the regulation of neuronal process formation and in differentiation of neural crest cells. May be down-regulated in neuroblastoma tumors. Substrate-specific adapter of an E3 ubiquitin-protein ligase complex which mediates the ubiquitination and subsequent proteasomal degradation of target proteins. -
Tissue specificity
Detected in fetal brain tissue, moderate expression in fetal heart, lung and kidney. Highly expressed in adult brain, particularly high in the hippocampus and amygdala, and spinal chord. Detectable in adult pancreas. -
Pathway
Protein modification; protein ubiquitination. -
Sequence similarities
Contains 1 BTB (POZ) domain.
Contains 6 Kelch repeats. -
Developmental stage
Dramatically up-regulated upon neuronal differentiation. -
Post-translational
modificationsUbiquitinated and probably targeted for proteasome-independent degradation. -
Cellular localization
Nucleus matrix. Cytoplasm > cytoskeleton. - Information by UniProt
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Database links
- Entrez Gene: 8507 Human
- Entrez Gene: 13803 Mouse
- Entrez Gene: 294674 Rat
- Omim: 605173 Human
- SwissProt: O14682 Human
- SwissProt: O35709 Mouse
- Unigene: 104925 Human
- Unigene: 241073 Mouse
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Alternative names
- CCL 28 antibody
- CCL28 antibody
- Ectoderm neural cortex protein 1 antibody
see all
Images
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Anti-ENC1 antibody [EPR5406(B)] (ab124902) at 1/1000 dilution + Human brain fetal lysate at 10 µg
Secondary
HRP labelled goat anti-rabbit at 1/2000 dilution
Predicted band size: 66 kDa
Observed band size: 66 kDa -
ab124902, at 1/100 dilution, staining ENC1 in paraffin-embedded Human brain tissue by Immunohistochemistry.
Perform heat mediated antigen retrieval before commencing with IHC staining protocol.
Protocols
Datasheets and documents
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SDS download
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Datasheet download
References (3)
ab124902 has been referenced in 3 publications.
- Wu C et al. Ectodermal-neural cortex 1 affects the biological function of lung cancer through the MAPK pathway. Int J Mol Med 47:N/A (2021). PubMed: 33693958
- Gong L et al. The mevalonate coordinates energy input and cell proliferation. Cell Death Dis 10:327 (2019). PubMed: 30975976
- Feng J et al. Identification of a subtype-specific ENC1 gene related to invasiveness in human pituitary null cell adenoma and oncocytomas. J Neurooncol 119:307-15 (2014). PubMed: 24916845