Key features and details
- Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
- Rabbit monoclonal [EPR5406(B)] to ENC1
- Suitable for: WB, IHC-P, ICC/IF
- Reacts with: Human
Product nameAnti-ENC1 antibody [EPR5406(B)]
See all ENC1 primary antibodies
DescriptionRabbit monoclonal [EPR5406(B)] to ENC1
Tested applicationsSuitable for: WB, IHC-P, ICC/IFmore details
Unsuitable for: IP
Species reactivityReacts with: Human
Predicted to work with: Mouse, Rat
Synthetic peptide within Human ENC1 aa 550-650. The exact sequence is proprietary.
- Human fetal brain lysate; Human brain tissue
This product is a recombinant monoclonal antibody, which offers several advantages including:
- - High batch-to-batch consistency and reproducibility
- - Improved sensitivity and specificity
- - Long-term security of supply
- - Animal-free production
Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.
Storage instructionsShipped at 4°C. Store at -20°C. Stable for 12 months at -20°C.
Dissociation constant (KD)KD = 1.03 x 10 -10 M Learn more about KD
Storage bufferpH: 7.20
Preservative: 0.05% Sodium azide
Constituents: 40% Glycerol, 9.85% Tris glycine, 50% Tissue culture supernatant
Concentration information loading...
PurityTissue culture supernatant
Our Abpromise guarantee covers the use of ab124902 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
|WB||1/1000 - 1/10000. Detects a band of approximately 66 kDa (predicted molecular weight: 66 kDa).|
|IHC-P||1/100 - 1/250. Perform heat mediated antigen retrieval before commencing with IHC staining protocol.|
|ICC/IF||1/50 - 1/100.|
FunctionActin-binding protein involved in the regulation of neuronal process formation and in differentiation of neural crest cells. May be down-regulated in neuroblastoma tumors. Substrate-specific adapter of an E3 ubiquitin-protein ligase complex which mediates the ubiquitination and subsequent proteasomal degradation of target proteins.
Tissue specificityDetected in fetal brain tissue, moderate expression in fetal heart, lung and kidney. Highly expressed in adult brain, particularly high in the hippocampus and amygdala, and spinal chord. Detectable in adult pancreas.
PathwayProtein modification; protein ubiquitination.
Sequence similaritiesContains 1 BTB (POZ) domain.
Contains 6 Kelch repeats.
Developmental stageDramatically up-regulated upon neuronal differentiation.
modificationsUbiquitinated and probably targeted for proteasome-independent degradation.
Cellular localizationNucleus matrix. Cytoplasm > cytoskeleton.
- Information by UniProt
- CCL 28 antibody
- CCL28 antibody
- Ectoderm neural cortex protein 1 antibody
Anti-ENC1 antibody [EPR5406(B)] (ab124902) at 1/1000 dilution + Human brain fetal lysate at 10 µg
HRP labelled goat anti-rabbit at 1/2000 dilution
Predicted band size: 66 kDa
Observed band size: 66 kDa
ab124902, at 1/100 dilution, staining ENC1 in paraffin-embedded Human brain tissue by Immunohistochemistry.
Perform heat mediated antigen retrieval before commencing with IHC staining protocol.
Equilibrium disassociation constant (KD)
Learn more about KD
Click here to learn more about KD
ab124902 has been referenced in 2 publications.
- Gong L et al. The mevalonate coordinates energy input and cell proliferation. Cell Death Dis 10:327 (2019). PubMed: 30975976
- Feng J et al. Identification of a subtype-specific ENC1 gene related to invasiveness in human pituitary null cell adenoma and oncocytomas. J Neurooncol 119:307-15 (2014). PubMed: 24916845