Overview

  • Product name

    Anti-ENC1 antibody [EPR5406(B)]
    See all ENC1 primary antibodies
  • Description

    Rabbit monoclonal [EPR5406(B)] to ENC1
  • Host species

    Rabbit
  • Tested applications

    Suitable for: WB, IHC-P, ICC/IFmore details
    Unsuitable for: IP
  • Species reactivity

    Reacts with: Human
    Predicted to work with: Mouse, Rat
  • Immunogen

    Synthetic peptide within Human ENC1 aa 550-650. The exact sequence is proprietary.

  • Positive control

    • Human fetal brain lysate; Human brain tissue
  • General notes

     

     

    Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMab® patents.

    This product is a recombinant rabbit monoclonal antibody.

Properties

Applications

Our Abpromise guarantee covers the use of ab124902 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB 1/1000 - 1/10000. Detects a band of approximately 66 kDa (predicted molecular weight: 66 kDa).
IHC-P 1/100 - 1/250. Perform heat mediated antigen retrieval before commencing with IHC staining protocol.
ICC/IF 1/50 - 1/100.
  • Application notes
    Is unsuitable for IP.
  • Target

    • Function

      Actin-binding protein involved in the regulation of neuronal process formation and in differentiation of neural crest cells. May be down-regulated in neuroblastoma tumors. Substrate-specific adapter of an E3 ubiquitin-protein ligase complex which mediates the ubiquitination and subsequent proteasomal degradation of target proteins.
    • Tissue specificity

      Detected in fetal brain tissue, moderate expression in fetal heart, lung and kidney. Highly expressed in adult brain, particularly high in the hippocampus and amygdala, and spinal chord. Detectable in adult pancreas.
    • Pathway

      Protein modification; protein ubiquitination.
    • Sequence similarities

      Contains 1 BTB (POZ) domain.
      Contains 6 Kelch repeats.
    • Developmental stage

      Dramatically up-regulated upon neuronal differentiation.
    • Post-translational
      modifications

      Ubiquitinated and probably targeted for proteasome-independent degradation.
    • Cellular localization

      Nucleus matrix. Cytoplasm > cytoskeleton.
    • Information by UniProt
    • Database links

    • Alternative names

      • CCL 28 antibody
      • CCL28 antibody
      • Ectoderm neural cortex protein 1 antibody
      • Ectoderm-neural cortex protein 1 antibody
      • Ectodermal neural cortex (with BTB like domain) antibody
      • ENC 1 antibody
      • ENC-1 antibody
      • ENC1 antibody
      • ENC1_HUMAN antibody
      • FLJ39259 antibody
      • Kelch like 35 antibody
      • Kelch like 37 antibody
      • Kelch-like protein 37 antibody
      • KLHL 35 antibody
      • KLHL35 antibody
      • KLHL37 antibody
      • NRPB antibody
      • Nuclear matrix protein NRP/B antibody
      • Nuclear restricted protein BTB domain like (brain) antibody
      • OTTHUMP00000128235 antibody
      • OTTHUMP00000222054 antibody
      • p53 induced gene 10 protein antibody
      • p53-induced gene 10 protein antibody
      • PIG 10 antibody
      • PIG10 antibody
      • TP53I10 antibody
      • Tumor protein p53 inducible protein 10 antibody
      see all

    Images

    • Anti-ENC1 antibody [EPR5406(B)] (ab124902) at 1/1000 dilution + Human brain fetal lysate at 10 µg

      Secondary
      HRP labelled goat anti-rabbit at 1/2000 dilution

      Predicted band size: 66 kDa
      Observed band size: 66 kDa

    • ab124902, at 1/100 dilution, staining ENC1 in paraffin-embedded Human brain tissue by Immunohistochemistry.
    • Equilibrium disassociation constant (KD)
      Learn more about KD

      Click here to learn more about KD

    References

    This product has been referenced in:

    • Gong L  et al. The mevalonate coordinates energy input and cell proliferation. Cell Death Dis 10:327 (2019). Read more (PubMed: 30975976) »
    • Feng J  et al. Identification of a subtype-specific ENC1 gene related to invasiveness in human pituitary null cell adenoma and oncocytomas. J Neurooncol 119:307-15 (2014). Read more (PubMed: 24916845) »
    See all 2 Publications for this product

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    Please note: All products are "FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC PROCEDURES"
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