Validated using a knockout cell line

Recombinant Anti-Endo G antibody [EP1665Y] (ab76122)


  • Product name

    Anti-Endo G antibody [EP1665Y]
    See all Endo G primary antibodies
  • Description

    Rabbit monoclonal [EP1665Y] to Endo G
  • Host species

  • Tested applications

    Suitable for: WBmore details
    Unsuitable for: Flow Cyt,ICC,IHC-P or IP
  • Species reactivity

    Reacts with: Mouse, Rat, Human
  • Immunogen

    Synthetic peptide within Human Endo G (C terminal). The exact sequence is proprietary.

  • Positive control

    • HepG2 whole cell lysate (ab7900)
  • General notes



    Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMab® patents.

    This product is a recombinant rabbit monoclonal antibody.



Our Abpromise guarantee covers the use of ab76122 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB 1/2000 - 1/5000. Detects a band of approximately 35 kDa (predicted molecular weight: 36 kDa).
  • Application notes
    Is unsuitable for Flow Cyt,ICC,IHC-P or IP.
  • Target

    • Relevance

      Endo G is a nuclear encoded endonuclease that is localized in the mitochondrion. The encoded protein cleaves DNA at GC tracts. It is capable of generating the RNA primers required by DNA polymerase gamma to initiate replication of mitochondrial DNA.
    • Cellular localization

      Cytoplasmic, Mitochondrial and Nuclear
    • Database links

    • Alternative names

      • EndoG antibody
      • Endonuclease G antibody
      • Endonuclease G mitochondrial antibody
      • EndonucleaseG antibody
      • FLJ27463 antibody
      • Mitochondrial endonuclease G antibody
      • NUCG_HUMAN antibody
      see all


    • Lane 1: Wild-type HAP1 cell lysate (20 µg)
      Lane 2: Endo G HAP1 cell lysate (20 µg)
      Lane 3: Mouse liver tissue lysate (20 µg)
      Lane 4: HepG2 cell lysate (20 µg)
      Lanes 1 - 4: Merged signal (red and green). Green - ab76122 observed at 29 kDa. Red - loading control, ab7291, observed at 52 kDa.

      ab76122 was shown to specifically react with Endo G when Endo G knockout samples were used. Wild-type and Endo G knockout samples were subjected to SDS-PAGE. ab76122 and ab7291 (loading control to alpha tubulin) were diluted 1/2000 and 1/10000 respectively and incubated overnight at 4°C. Blots were developed with Goat anti-Rabbit IgG H&L (IRDye® 800CW) preadsorbed (ab216773) and Goat anti-Mouse IgG H&L (IRDye® 680RD)preadsorbed (ab216776) secondary antibodies at 1/10000 dilution for 1 h at room temperature before imaging.

    • Anti-Endo G antibody [EP1665Y] (ab76122) at 1/2000 dilution + HepG2 cell lysate at 10 µg

      HRP labelled goat anti-rabbit at 1/1000 dilution

      Developed using the ECL technique.

      Predicted band size: 36 kDa
      Observed band size: 35 kDa
      why is the actual band size different from the predicted?


    This product has been referenced in:

    • Pardo R  et al. EndoG Knockout Mice Show Increased Brown Adipocyte Recruitment in White Adipose Tissue and Improved Glucose Homeostasis. Endocrinology 157:3873-3887 (2016). Mouse . Read more (PubMed: 27547848) »
    See 1 Publication for this product

    Customer reviews and Q&As


    Thank you for confirming these details and for your cooperation. The details provided enable us to closely monitor the quality of our products.

    I am sorry this product did not perform as stated on the datasheet and for the inconvenience this has caused. As requested, I have issued a free of charge for one vial of ab7900replacement with the order number 1148322.

    Expect to receive this product on Tuesday August 21. To check the status of the order please contact our Customer Service team and reference this number.

    Please note that this free of charge replacement vial is also covered by our Abpromise guarantee. Should you still be experiencing difficulties, or if you have any further questions, please do not hesitate to let us know.

    I wish you the best of luck with your research.

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