Product nameEndogenous Avidin + Biotin Blocking System
See all Endogenous Avidin+Biotin Blocking System kits
The purpose is to decrease the signal caused by endogenous avidin, biotin, or biotin-binding proteins when immunostaining specimens with detection systems that rely on avidin-biotin / steptavidin-biotin (ABC systems). Non-specific staining due to these factors commonly resembles a diffuse, weak, cytoplasmic pattern.
Tested applicationsSuitable for: IHC-Fr, IHC-P, ICC/IFmore details
Storage instructionsStore at +4°C. Please refer to protocols.
Storage bufferPreservative: 0.1% Sodium Azide in both components
Components 1 kit Avidin solution 1 x 15ml Biotin Solution 1 x 15ml
RelevanceSome cells, and tissues such as kidney, liver and spleen, contain endogenous biotin. Using an avidin-biotin staining method may result in high, non-specific background staining. A significant reduction of this non-specific background can be obtained by pre-treatment of cells/tissues with avidin/biotin blocking reagents prior to the incubation of biotinylated antibody.
Our Abpromise guarantee covers the use of ab3387 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
|IHC-Fr||Use at an assay dependent dilution.|
|IHC-P||Use at an assay dependent dilution.|
|ICC/IF||Use at an assay dependent dilution.|
This product has been referenced in:
- Liakhovitskaia A et al. Runx1 is required for progression of CD41+ embryonic precursors into HSCs but not prior to this. Development 141:3319-23 (2014). IHC-Fr ; Mouse . Read more (PubMed: 25139854) »
- Vesterdal LK et al. Accumulation of lipids and oxidatively damaged DNA in hepatocytes exposed to particles. Toxicol Appl Pharmacol N/A:N/A (2013). Read more (PubMed: 24121055) »