Endogenous Avidin + Biotin Blocking System (ab3387)


  • Product name
    Endogenous Avidin + Biotin Blocking System
    See all Endogenous Avidin+Biotin Blocking System kits
  • Notes

    The purpose is to decrease the signal caused by endogenous avidin, biotin, or biotin-binding proteins when immunostaining specimens with detection systems that rely on avidin-biotin / steptavidin-biotin (ABC systems). Non-specific staining due to these factors commonly resembles a diffuse, weak, cytoplasmic pattern.

  • Tested applications
    Suitable for: IHC-Fr, IHC-P, ICC/IFmore details


  • Storage instructions
    Store at +4°C. Please refer to protocols.
  • Storage buffer
    Preservative: 0.1% Sodium Azide in both components
  • Components 1 kit
    Avidin solution 1 x 15ml
    Biotin Solution 1 x 15ml
  • Research areas
  • Relevance
    Some cells, and tissues such as kidney, liver and spleen, contain endogenous biotin. Using an avidin-biotin staining method may result in high, non-specific background staining. A significant reduction of this non-specific background can be obtained by pre-treatment of cells/tissues with avidin/biotin blocking reagents prior to the incubation of biotinylated antibody.


Our Abpromise guarantee covers the use of ab3387 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
IHC-Fr Use at an assay dependent dilution.
IHC-P Use at an assay dependent dilution.
ICC/IF Use at an assay dependent dilution.


This product has been referenced in:
  • Liakhovitskaia A  et al. Runx1 is required for progression of CD41+ embryonic precursors into HSCs but not prior to this. Development 141:3319-23 (2014). IHC-Fr ; Mouse . Read more (PubMed: 25139854) »
  • Vesterdal LK  et al. Accumulation of lipids and oxidatively damaged DNA in hepatocytes exposed to particles. Toxicol Appl Pharmacol N/A:N/A (2013). Read more (PubMed: 24121055) »

See all 3 Publications for this product

Customer reviews and Q&As

We tested this product either with fixed cells or with tissue sections in immunocytochemistry or Immunohistochemistry applications, living cells unfortunately were never tested. There are two main things to consider while using this product with livi...

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We do not have set expiration dates for our products. Most antibodies are stable and can last for anywhere from a few months to several years if stored properly, so we strongly recommend that you follow the storage instructions on the datasheet for the...

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Thank you for your reply.

I would suggest changing your antigen retrieval method next. While you have used sodium citrate pH 6 and Tris/EDTA pH 10 I would recommend the following procedure based on a paper from Jiao et al which I will include...

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I am sorry to hear of the troubles which you have been experiencing in IHC-P. In reviewing the protocol and troubleshooting provided I did not see that you block endogenous avidin or biotin. We highly recommend bl...

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Thank you for contacting us regarding this matter.

We have updated the MSDS and have fixed this issue. We would like to thank you for bringing this to our attention. I gave included the updated MSDS here but it is available on the webpage for ...

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Thank you for your inquiry. My colleague is not in the office today and I am happy to answer your question instead of her. Please find below a protocol for blocking biotin with egg white:
1- Wash twice in TBS 0.05M pH7.5, to which 0.01% Tween 20 h...

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Thank you for contacting us.
Please be aware that the biotin problem is still not solved by using milk or BSA: those two will block the non-specific protein binding sites, but not the endogenous biotin. You can block the endogenous biotin by using...

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Is the UV block from DAKO a protein block? We typically recommend performing a protein block using 10% normal serum from the host species of your secondary antibody. For example, if your secondary is goat anti-rab...

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Thank you for contacting us. I'm glad to hear you have been getting good staining with ab469. In order to help you obtain more intense staining would you mind filling in the form which I have attached to this email. It will allow me to more fully unde...

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Vielen Dank für Ihre Anfrage und dafür, dass Sie sich die Zeit genommen haben, unseren Fragebogen auszufüllen. Es tut mir leid, dass Sie Probleme mit unserem VEGF-Antikörper ab3109 hatten. Ich weiß, dass Sie schon viel Zeit in Ihre Experimente inves...

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