Product nameEndogenous Avidin/Biotin Blocking Kit
See all Endogenous Avidin+Biotin Blocking System kits
Avidin Biotin Blocking Kit ab64212 blocks signal from endogenous avidin, biotin and biotin-binding proteins in tissues when used with biotin-based IHC detection (eg. with ABC IHC detection kits).
Find more kits and reagents for antigen retrieval, blocking, signal amplification, visualization, counterstaining, and mounting in the IHC kits and reagents guide.
Tested applicationsSuitable for: IHC-P, ICC/IF, IHC-Frmore details
Storage instructionsStore at +4°C. Please refer to protocols.
Storage bufferPreservative: 0.08% Sodium azide
Components 15 ml 15 ml Avidin Block 1 x 15ml 1 x 15ml Biotin Block 1 x 15ml 1 x 15ml
RelevanceSome cells, and tissues such as kidney, liver and spleen, contain endogenous biotin. Using an avidin-biotin staining method may result in high, non-specific background staining. A significant reduction of this non-specific background can be obtained by pre-treatment of cells/tissues with avidin/biotin blocking reagents prior to the incubation of biotinylated antibody.
Our Abpromise guarantee covers the use of ab64212 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
|IHC-P||Use at an assay dependent concentration.|
|ICC/IF||Use at an assay dependent concentration.|
|IHC-Fr||Use at an assay dependent concentration.|
Immunohistochemical analysis staining HTRA1 in mouse bone tissue sections. Tissue sections were dewaxed, rehydrated and treated with Endogenous Avidin/Biotin Blocking Kit (ab64212), 3% H2O2 and normal swine serum. Tissue sections were then incubated with a polyclonal anti-HTRA1 antibody for 1 hour at 37°C. After PBS wash, tissue sections are incubated with biotinylated swine anti-rabbit IgG for 45 minutes at 37°C and further incubated for 30 minutes after washing. With Vectastatin. Sections were developed using DAB and counterstained using Harris modified hematoxylin.
This product has been referenced in:
- Hammad S et al. Confounding influence of tamoxifen in mouse models of Cre recombinase-induced gene activity or modulation. Arch Toxicol 92:2549-2561 (2018). Read more (PubMed: 29974145) »
- Filliat G et al. Role of HTRA1 in bone formation and regeneration: In vitro and in vivo evaluation. PLoS One 12:e0181600 (2017). IHC . Read more (PubMed: 28732055) »