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In the demonstration of tissue antigens by Immunohistochemistry, the evaluation of specific staining is sometimes impeded by the presence of endogenous biotin, which may lead to an erroneous interpretation or false positive results. This blocking system is intended for use in immunohistochemical staining to block nonspecific staining due to endogenous biotin during the IHC detection of antigens.
|Components||Identifier||15 ml||15 ml|
|Biotin Block||—||1 x 15ml||1 x 15ml|
Our Abpromise guarantee covers the use of ab64212 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
|IHC-P||Use at an assay dependent concentration.|
|ICC/IF||Use at an assay dependent concentration.|
|IHC-Fr||Use at an assay dependent concentration.|
Immunohistochemical analysis staining HTRA1 in mouse bone tissue sections. Tissue sections were dewaxed, rehydrated and treated with Endogenous Avidin/Biotin Blocking Kit (ab64212), 3% H2O2 and normal swine serum. Tissue sections were then incubated with a polyclonal anti-HTRA1 antibody for 1 hour at 37°C. After PBS wash, tissue sections are incubated with biotinylated swine anti-rabbit IgG for 45 minutes at 37°C and further incubated for 30 minutes after washing. With Vectastatin. Sections were developed using DAB and counterstained using Harris modified hematoxylin.
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