Overview

  • Product name
    Endogenous Avidin/Biotin Blocking Kit
    See all Endogenous Avidin+Biotin Blocking System kits
  • Product overview

    Avidin Biotin Blocking Kit ab64212 blocks signal from endogenous avidin, biotin and biotin-binding proteins in tissues when used with biotin-based IHC detection (eg. with ABC IHC detection kits).


    Find more kits and reagents for antigen retrieval, blocking, signal amplification, visualization, counterstaining, and mounting in the IHC kits and reagents guide.


     

  • Tested applications
    Suitable for: IHC-P, ICC/IF, IHC-Frmore details

Properties

  • Storage instructions
    Store at +4°C. Please refer to protocols.
  • Storage buffer
    Preservative: 0.08% Sodium azide
    Constituent: Avidin
  • Components 15 ml
    Avidin Block 1 x 15ml
    Biotin Block 1 x 15ml
  • Research areas
  • Relevance
    Some cells, and tissues such as kidney, liver and spleen, contain endogenous biotin. Using an avidin-biotin staining method may result in high, non-specific background staining. A significant reduction of this non-specific background can be obtained by pre-treatment of cells/tissues with avidin/biotin blocking reagents prior to the incubation of biotinylated antibody.

Applications

Our Abpromise guarantee covers the use of ab64212 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
IHC-P Use at an assay dependent concentration.
ICC/IF Use at an assay dependent concentration.
IHC-Fr Use at an assay dependent concentration.

Images

  • Immunohistochemical analysis staining HTRA1 in mouse bone tissue sections. Tissue sections were dewaxed, rehydrated and treated with Endogenous Avidin/Biotin Blocking Kit (ab64212), 3% H2Oand normal swine serum. Tissue sections were then incubated with a polyclonal anti-HTRA1 antibody for 1 hour at 37°C. After PBS wash, tissue sections are incubated with biotinylated swine anti-rabbit IgG for 45 minutes at 37°C and further incubated for 30 minutes after washing. With Vectastatin. Sections were developed using DAB and counterstained using Harris modified hematoxylin. 

References

This product has been referenced in:
  • Peng C  et al. Synchronous primary sigmoid colon cancer and primary thyroid cancer followed by a malignant tumor of the kidney: Case report of multiple primary cancer and review of the literature. Oncol Lett 17:2479-2484 (2019). Read more (PubMed: 30719116) »
  • Teissier T  et al. Knockout of receptor for advanced glycation end-products attenuates age-related renal lesions. Aging Cell 18:e12850 (2019). Read more (PubMed: 30794349) »
See all 6 Publications for this product

Customer reviews and Q&As

Filter by Ratings

helpful kit

Excellent Excellent 5/5 (Ease of Use)
Abreviews
We used this kit to block the non-specific binding of avidin or biotinylated reagents with the endogenous biotin, also their receptors and the avidin binding sites in the tissue.
Its works good and is easy to use.
We used the kit as a ready to use-kit and incubated the samples first with the avidin block for 10minutes and after a washing step with the bioin block for 10minutes.

Abcam user community

Verified customer

Submitted Mar 12 2018

Please note: All products are "FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC PROCEDURES"
For licensing inquiries, please contact partnerships@abcam.com

Sign up