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RecombinantRabMAb

Recombinant Anti-ENDOGL1/ENGL antibody [EPR15672] - BSA and Azide free (ab251450)

  • Datasheet
  • Certificate of Compliance
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Western blot - Anti-ENDOGL1/ENGL antibody [EPR15672] - BSA and Azide free (ab251450)
  • Western blot - Anti-ENDOGL1/ENGL antibody [EPR15672] - BSA and Azide free (ab251450)
  • Immunoprecipitation - Anti-ENDOGL1/ENGL antibody [EPR15672] - BSA and Azide free (ab251450)
  • Anti-ENDOGL1/ENGL antibody [EPR15672] - BSA and Azide free (ab251450)

Key features and details

  • Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
  • Rabbit monoclonal [EPR15672] to ENDOGL1/ENGL - BSA and Azide free
  • Suitable for: WB, IP
  • Reacts with: Human

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Conjugation
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HRP Conjugation Kit - Lightning-Link® (ab102890)
Protein
Product image
Recombinant Human ENDOGL1/ENGL protein (ab160724)

View more associated products

Overview

  • Product name

    Anti-ENDOGL1/ENGL antibody [EPR15672] - BSA and Azide free
    See all ENDOGL1/ENGL primary antibodies
  • Description

    Rabbit monoclonal [EPR15672] to ENDOGL1/ENGL - BSA and Azide free
  • Host species

    Rabbit
  • Tested Applications & Species

    Application Species
    IP
    Human
    WB
    Human
    See all applications and species data
  • Immunogen

    Synthetic peptide. This information is proprietary to Abcam and/or its suppliers.

  • General notes

    ab251450 is the carrier-free version of ab206300 This format is designed for use in antibody labeling, including fluorochromes, metal isotopes, oligonucleotides, enzymes.

     

    Our carrier-free formats are supplied in a buffer free of BSA, sodium azide and glycerol for higher conjugation efficiency.

    Use our conjugation kits  for antibody conjugates that are ready-to-use in as little as 20 minutes with <1 minute hands-on-time and 100% antibody recovery: available for fluorescent dyes, HRP, biotin and gold.

    Ab251450 is compatible with the Maxpar® Antibody Labeling Kit from Fluidigm.

    Maxpar® is a trademark of Fluidigm Canada Inc.

    This product was previously labelled as ENDOGL1

    This product is a recombinant monoclonal antibody, which offers several advantages including:

    • - High batch-to-batch consistency and reproducibility
    • - Improved sensitivity and specificity
    • - Long-term security of supply
    • - Animal-free production
    For more information see here.

    Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.

Properties

  • Form

    Liquid
  • Storage instructions

    Shipped at 4°C. Store at +4°C. Do Not Freeze.
  • Storage buffer

    pH: 7.2
    Constituent: PBS
  • Carrier free

    Yes
  • Concentration information loading...
  • Clonality

    Monoclonal
  • Clone number

    EPR15672
  • Isotype

    IgG
  • Research areas

    • Tags & Cell Markers
    • Subcellular Markers
    • Organelles
    • Mitochondria
    • Epigenetics and Nuclear Signaling
    • Chromatin Binding Proteins
    • DNA / RNA binding
    • Metabolism
    • Pathways and Processes
    • Mitochondrial Metabolism
    • Mitochondrial markers

Associated products

  • Alternative Versions

    • Anti-ENDOGL1/ENGL antibody [EPR15672] (ab206300)
  • Compatible Secondaries

    • Goat Anti-Rabbit IgG H&L (HRP) (ab97051)
  • Conjugation kits

    • HRP Conjugation Kit - Lightning-Link® (ab102890)
  • Recombinant Protein

    • Recombinant Human ENDOGL1/ENGL protein (ab160724)
  • Related Products

    • VeriBlot for IP Detection Reagent (HRP) (ab131366)

Applications

The Abpromise guarantee

Our Abpromise guarantee covers the use of ab251450 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Guaranteed

Tested applications are guaranteed to work and covered by our Abpromise guarantee.

Predicted

Predicted to work for this combination of applications and species but not guaranteed.

Incompatible

Does not work for this combination of applications and species.

Application Species
IP
Human
WB
Human
Application Abreviews Notes
WB
Use at an assay dependent concentration. Detects a band of approximately 41 kDa (predicted molecular weight: 41 kDa).
IP
Use at an assay dependent concentration.
Notes
WB
Use at an assay dependent concentration. Detects a band of approximately 41 kDa (predicted molecular weight: 41 kDa).
IP
Use at an assay dependent concentration.

Target

  • Function

    Endo/exonuclease with nicking activity towards supercoiled DNA, a preference for single stranded DNA and 5'-3' exonuclease activity.
  • Tissue specificity

    Ubiquitous.
  • Sequence similarities

    Belongs to the DNA/RNA non-specific endonuclease family.
  • Cellular localization

    Mitochondrion inner membrane.
  • Target information above from: UniProt accession Q9Y2C4 The UniProt Consortium
    The Universal Protein Resource (UniProt) in 2010
    Nucleic Acids Res. 38:D142-D148 (2010) .

    Information by UniProt
  • Database links

    • Entrez Gene: 9941 Human
    • Omim: 604051 Human
    • SwissProt: Q9Y2C4 Human
    • Unigene: 517897 Human
    • Alternative names

      • Endo G like 1 antibody
      • Endo G-like 1 antibody
      • Endo/exonuclease (5'-3'), endonuclease G like antibody
      • ENDOGL 1 antibody
      • ENDOGL1 antibody
      • ENDOGL2 antibody
      • Endonuclease G like 1 antibody
      • Endonuclease G like 2 antibody
      • Endonuclease G-like 1 antibody
      • ENGL a antibody
      • ENGL antibody
      • ENGL B antibody
      • ENGLA antibody
      • ENGLB antibody
      • Exog antibody
      • EXOG_HUMAN antibody
      • MGC125944 antibody
      • MGC125945 antibody
      • mitochondrial antibody
      • Nuclease EXOG antibody
      • Nuclease EXOG mitochondrial antibody
      see all

    Images

    • Western blot - Anti-ENDOGL1/ENGL antibody [EPR15672] - BSA and Azide free (ab251450)
      Western blot - Anti-ENDOGL1/ENGL antibody [EPR15672] - BSA and Azide free (ab251450)
      All lanes : Anti-ENDOGL1/ENGL antibody [EPR15672] (ab206300) at 1/1000 dilution

      Lane 1 : Human fetal heart tissue lysate
      Lane 2 : Human fetal kidney tissue lysate

      Lysates/proteins at 10 µg per lane.

      Secondary
      All lanes : Anti-Rabbit IgG (HRP), specific to the non-reduced form of IgG at 1/10000 dilution

      Predicted band size: 41 kDa
      Observed band size: 41 kDa


      Exposure time: 3 minutes


      This data was developed using ab206300, the same antibody clone in a different buffer formulation.

      Blocking and dilution buffer: 5% NFDM/TBST.

    • Western blot - Anti-ENDOGL1/ENGL antibody [EPR15672] - BSA and Azide free (ab251450)
      Western blot - Anti-ENDOGL1/ENGL antibody [EPR15672] - BSA and Azide free (ab251450)
      All lanes : Anti-ENDOGL1/ENGL antibody [EPR15672] (ab206300) at 1/1000 dilution

      Lane 1 : HeLa (Human epithelial cells from cervix adenocarcinoma) whole cell lysate
      Lane 2 : HepG2 (Human liver hepatocellular carcinoma) whole cell lysate
      Lane 3 : HUVEC (Human umbilical vein endothelial cell line) whole cell lysate

      Lysates/proteins at 20 µg per lane.

      Secondary
      All lanes : Goat Anti-Rabbit IgG H&L (HRP) (ab97051) at 1/100000 dilution

      Predicted band size: 41 kDa
      Observed band size: 41 kDa


      Exposure time: 3 minutes


      This data was developed using ab206300, the same antibody clone in a different buffer formulation.

      Blocking and dilution buffer: 5% NFDM/TBST.

    • Immunoprecipitation - Anti-ENDOGL1/ENGL antibody [EPR15672] - BSA and Azide free (ab251450)
      Immunoprecipitation - Anti-ENDOGL1/ENGL antibody [EPR15672] - BSA and Azide free (ab251450)
      This data was developed using ab206300, the same antibody clone in a different buffer formulation.ENDOGL1/ENGL was immunoprecipitated from 1mg of HUVEC (Human umbilical vein endothelial cell line) whole cell lysate with ab206300 at 1/40 dilution. Western blot was performed from the immunoprecipitate using ab206300 at 1/1000 dilution. VeriBlot for IP Detection Reagent (HRP) (ab131366), was used for detection at 1/10000 dilution. Lane 1: HUVEC whole cell lysate 10ug (Input). Lane 2: ab206300 IP in HUVEC whole cell lysate. Lane 3: Rabbit monoclonal IgG (ab172730) instead of ab206300 in HUVEC whole cell lysate. Blocking and dilution buffer and concentration: 5% NFDM/TBST. Exposure time: 10 seconds.
    • Anti-ENDOGL1/ENGL antibody [EPR15672] - BSA and Azide free (ab251450)
      Anti-ENDOGL1/ENGL antibody [EPR15672] - BSA and Azide free (ab251450)

    Protocols

    To our knowledge, customised protocols are not required for this product. Please try the standard protocols listed below and let us know how you get on.

    Click here to view the general protocols

    Datasheets and documents

    • Datasheet

    Certificate of Compliance

    To download a Certificate of Compliance, please enter your Lot number below:

  • References (0)

    Publishing research using ab251450? Please let us know so that we can cite the reference in this datasheet.

    ab251450 has not yet been referenced specifically in any publications.

    Customer reviews and Q&As

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