Recombinant Anti-ENDOGL1/ENGL antibody [EPR15672] - BSA and Azide free (ab251450)
![Western blot - Anti-ENDOGL1/ENGL antibody [EPR15672] - BSA and Azide free (ab251450)](https://www.abcam.com/ps/products/251/ab251450/Images/ab251450-406076-anti-endogl1-engl-antibody-epr15672-bsa-and-azide-free-western-blot.jpg "Western blot - Anti-ENDOGL1/ENGL antibody [EPR15672] - BSA and Azide free (ab251450)")
Key features and details
- Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
- Rabbit monoclonal [EPR15672] to ENDOGL1/ENGL - BSA and Azide free
- Suitable for: WB, IP
- Reacts with: Human
Overview
-
Product name
Anti-ENDOGL1/ENGL antibody [EPR15672] - BSA and Azide free
See all ENDOGL1/ENGL primary antibodies -
Description
Rabbit monoclonal [EPR15672] to ENDOGL1/ENGL - BSA and Azide free -
Host species
Rabbit -
Tested Applications & Species
See all applications and species dataApplication Species IP HumanWB Human
-
Immunogen
Synthetic peptide. This information is proprietary to Abcam and/or its suppliers.
-
General notes
ab251450 is the carrier-free version of ab206300 This format is designed for use in antibody labeling, including fluorochromes, metal isotopes, oligonucleotides, enzymes.
Our carrier-free formats are supplied in a buffer free of BSA, sodium azide and glycerol for higher conjugation efficiency.
Use our conjugation kits for antibody conjugates that are ready-to-use in as little as 20 minutes with <1 minute hands-on-time and 100% antibody recovery: available for fluorescent dyes, HRP, biotin and gold.
Ab251450 is compatible with the Maxpar® Antibody Labeling Kit from Fluidigm.
Maxpar® is a trademark of Fluidigm Canada Inc.
This product was previously labelled as ENDOGL1
This product is a recombinant monoclonal antibody, which offers several advantages including:
- - High batch-to-batch consistency and reproducibility
- - Improved sensitivity and specificity
- - Long-term security of supply
- - Animal-free production
Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.
Properties
-
Form
Liquid -
Storage instructions
Shipped at 4°C. Store at +4°C. Do Not Freeze. -
Storage buffer
pH: 7.2
Constituent: PBS -
Carrier free
Yes -
Concentration information loading... -
Clonality
Monoclonal -
Clone number
EPR15672 -
Isotype
IgG -
Research areas
Associated products
-
Alternative Versions
-
Compatible Secondaries
-
Conjugation kits
-
Recombinant Protein
-
Related Products
Applications
The Abpromise guarantee
Our Abpromise guarantee covers the use of ab251450 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
Tested applications are guaranteed to work and covered by our Abpromise guarantee.
Predicted to work for this combination of applications and species but not guaranteed.
Does not work for this combination of applications and species.
| Application | Species |
|---|---|
| IP |
Human
|
| WB |
Human
|
| Application | Abreviews | Notes |
|---|---|---|
| WB |
Use at an assay dependent concentration. Detects a band of approximately 41 kDa (predicted molecular weight: 41 kDa).
|
|
| IP |
Use at an assay dependent concentration.
|
| Notes |
|---|
|
WB
Use at an assay dependent concentration. Detects a band of approximately 41 kDa (predicted molecular weight: 41 kDa). |
|
IP
Use at an assay dependent concentration. |
Target
-
Function
Endo/exonuclease with nicking activity towards supercoiled DNA, a preference for single stranded DNA and 5'-3' exonuclease activity. -
Tissue specificity
Ubiquitous. -
Sequence similarities
Belongs to the DNA/RNA non-specific endonuclease family. -
Cellular localization
Mitochondrion inner membrane. - Information by UniProt
-
Database links
- Entrez Gene: 9941 Human
- Omim: 604051 Human
- SwissProt: Q9Y2C4 Human
- Unigene: 517897 Human
-
Alternative names
- Endo G like 1 antibody
- Endo G-like 1 antibody
- Endo/exonuclease (5'-3'), endonuclease G like antibody
see all
Images
-
Western blot - Anti-ENDOGL1/ENGL antibody [EPR15672] - BSA and Azide free (ab251450)All lanes : Anti-ENDOGL1/ENGL antibody [EPR15672] (ab206300) at 1/1000 dilution
Lane 1 : Human fetal heart tissue lysate
Lane 2 : Human fetal kidney tissue lysate
Lysates/proteins at 10 µg per lane.
Secondary
All lanes : Anti-Rabbit IgG (HRP), specific to the non-reduced form of IgG at 1/10000 dilution
Predicted band size: 41 kDa
Observed band size: 41 kDa
Exposure time: 3 minutesThis data was developed using ab206300, the same antibody clone in a different buffer formulation.
Blocking and dilution buffer: 5% NFDM/TBST.
-
![Western blot - Anti-ENDOGL1/ENGL antibody [EPR15672] - BSA and Azide free (ab251450)](https://www.abcam.com/ps/products/251/ab251450/Images/ab251450-403403-anti-endogl1-engl-antibody-epr15672-bsa-and-azide-free-western-blot.jpg)
Western blot - Anti-ENDOGL1/ENGL antibody [EPR15672] - BSA and Azide free (ab251450)All lanes : Anti-ENDOGL1/ENGL antibody [EPR15672] (ab206300) at 1/1000 dilution
Lane 1 : HeLa (Human epithelial cells from cervix adenocarcinoma) whole cell lysate
Lane 2 : HepG2 (Human liver hepatocellular carcinoma) whole cell lysate
Lane 3 : HUVEC (Human umbilical vein endothelial cell line) whole cell lysate
Lysates/proteins at 20 µg per lane.
Secondary
All lanes : Goat Anti-Rabbit IgG H&L (HRP) (ab97051) at 1/100000 dilution
Predicted band size: 41 kDa
Observed band size: 41 kDa
Exposure time: 3 minutesThis data was developed using ab206300, the same antibody clone in a different buffer formulation.
Blocking and dilution buffer: 5% NFDM/TBST.
-
![Immunoprecipitation - Anti-ENDOGL1/ENGL antibody [EPR15672] - BSA and Azide free (ab251450)](https://www.abcam.com/ps/products/251/ab251450/Images/ab251450-1-anti-endogl1-engl-antibody-epr15672-bsa-and-azide-free-immunoprecipitation.jpg)
Immunoprecipitation - Anti-ENDOGL1/ENGL antibody [EPR15672] - BSA and Azide free (ab251450)This data was developed using ab206300, the same antibody clone in a different buffer formulation.ENDOGL1/ENGL was immunoprecipitated from 1mg of HUVEC (Human umbilical vein endothelial cell line) whole cell lysate with ab206300 at 1/40 dilution. Western blot was performed from the immunoprecipitate using ab206300 at 1/1000 dilution. VeriBlot for IP Detection Reagent (HRP) (ab131366), was used for detection at 1/10000 dilution. Lane 1: HUVEC whole cell lysate 10ug (Input). Lane 2: ab206300 IP in HUVEC whole cell lysate. Lane 3: Rabbit monoclonal IgG (ab172730) instead of ab206300 in HUVEC whole cell lysate. Blocking and dilution buffer and concentration: 5% NFDM/TBST. Exposure time: 10 seconds. -
![Anti-ENDOGL1/ENGL antibody [EPR15672] - BSA and Azide free (ab251450)](https://www.abcam.com/ps/products/251/ab251450/Images/ab251450-4-benefits-of-recombinant-antibodies.png)
Anti-ENDOGL1/ENGL antibody [EPR15672] - BSA and Azide free (ab251450)
Protocols
To our knowledge, customised protocols are not required for this product. Please try the standard protocols listed below and let us know how you get on.
Datasheets and documents
Certificate of Compliance
References (0)
ab251450 has not yet been referenced specifically in any publications.
