Key features and details
- Rabbit polyclonal to Endoglycan
- Suitable for: IP, WB
- Reacts with: Human
- Isotype: IgG
Product nameAnti-Endoglycan antibody
See all Endoglycan primary antibodies
DescriptionRabbit polyclonal to Endoglycan
Tested applicationsSuitable for: IP, WBmore details
Species reactivityReacts with: Human
Predicted to work with: Chimpanzee, Macaque monkey, Rhesus monkey, Orangutan
- HeLa and 293T whole cell lysates.
Storage instructionsShipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle.
Storage bufferpH: 7
Preservative: 0.09% Sodium azide
Constituent: 99% Tris citrate/phosphate
pH 7 to 8
Concentration information loading...
PurityImmunogen affinity purified
Our Abpromise guarantee covers the use of ab191173 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
|IP||Use at 2-10 µg/mg of lysate.|
|WB||1/2500 - 1/5000. Predicted molecular weight: 65 kDa.|
RelevanceEndoglycan is a disulfide-linked homodimeric membrane protein belongong to the podocalyxin family.
Cellular localizationMembrane; Single-pass type I membrane protein
- EG antibody
- OTTHUMP00000216064 antibody
- PODLX2 antibody
Immunoprecipitation of HeLa whole cell lysate using ab191173 (lane 1) at 6 µg per reaction (0.5 or 1 mg/IP; 20% of IP loaded/lane). Immunoblot analysis of Endoglycan utilised ab191173 at 1µg/ml. The lysate in lane 2 is precipitated with an IgG control antibody. Detection utilised chemiluminescence with an exposure time of 30seconds.
All lanes : Anti-Endoglycan antibody (ab191173) at 1 µg/ml
Lane 1 : HeLa whole cell lysate prepared using RIPA lysis buffer
Lane 2 : 293T whole cell lysate prepared using RIPA lysis buffer
Lane 3 : Jurkat whole cell lysate prepared using RIPA lysis buffer
Lysates/proteins at 50 µg per lane.
Developed using the ECL technique.
Predicted band size: 65 kDa
Exposure time: 30 seconds
ab191173 has not yet been referenced specifically in any publications.