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Our Abpromise guarantee covers the use of ab191173 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
|IP||Use at 2-10 µg/mg of lysate.|
|WB||1/2500 - 1/5000. Predicted molecular weight: 65 kDa.|
Immunoprecipitation of HeLa whole cell lysate using ab191173 (lane 1) at 6 µg per reaction (0.5 or 1 mg/IP; 20% of IP loaded/lane). Immunoblot analysis of Endoglycan utilised ab191173 at 1µg/ml. The lysate in lane 2 is precipitated with an IgG control antibody. Detection utilised chemiluminescence with an exposure time of 30seconds.
ab191173 has not yet been referenced specifically in any publications.
Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"