Anti-Endomorphin 1 antibody (ab10285)

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Overview

  • Product name
    Anti-Endomorphin 1 antibody
    See all Endomorphin 1 primary antibodies
  • Description
    Rabbit polyclonal to Endomorphin 1
  • Host species
    Rabbit
  • Specificity
    Sensitivity has been determined by ELISA. Less than 1ng of antibody could be detected after binding to Endomorphin 1 which is conjugated to titer plate: Endomorphin 1 = 100 Endomorphin 2 = 5 Leu5-Enkephalin = <0.01 Met5-Enkephalin = <0.03 beta-Endorphin = <0.01
  • Tested applications
    Suitable for: IHC-Fr, ELISAmore details
  • Species reactivity
    Reacts with: Mouse, Rat, Human, Pig, Monkey
  • Immunogen

    Synthetic peptide conjugated to BSA.

Properties

  • Form
    Liquid
  • Storage instructions
    Shipped at 4°C. Upon delivery aliquot and store at -20°C or -80°C. Avoid repeated freeze / thaw cycles.
  • Concentration information loading...
  • Purity
    Immunogen affinity purified
  • Purification notes
    Selectivity towards Endomorphin 1 has been achieved by separating fraction of affinity purified antibodies which did not bind to Endomorphin 2.
  • Clonality
    Polyclonal
  • Isotype
    IgG
  • Research areas

Applications

Our Abpromise guarantee covers the use of ab10285 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
IHC-Fr Use a concentration of 15 µg/ml.
ELISA Use at an assay dependent concentration.

Target

  • Relevance
    The endogenous agonists for the mu-opiate receptors, endomorphin-1; Tyr-Pro-Trp-Phe-NH2, and endomorphin-2; Tyr-Pro-Phe-Phe-NH2 have been reported to show hypotensive activity and Ca2+ channel current inhibitory activity [Biochem. Biophys. Res. Commun.,235, 567 (1997)]. Although first identified in bovine brain, it is thought that endomorphin-1 and 2 are common mu-opiate agonists in mammals, including humans. Endomorphins may be useful for the study of pain perception together with some other biological functions in the body.
  • Alternative names
    • EM 1 antibody

Images

  • Immunohistochemistry (Frozen sections) - Anti-Endomorphin 1 antibody (ab10285)
    Immunohistochemistry (Frozen sections) - Anti-Endomorphin 1 antibody (ab10285)
    ab10285 staining Endomorphin 1 in rat Peryaqueductal Grey Matter by Immunohistochemistry (PFA perfusion fixed frozen sections).

References

This product has been referenced in:
  • Sun GC  et al. GPCR dimerization in brainstem nuclei contributes to the development of hypertension. Br J Pharmacol 172:2507-18 (2015). IHC ; Rat . Read more (PubMed: 25573074) »
See 1 Publication for this product

Publishing research using ab10285? Please let us know so that we can cite the reference in this datasheet.

Customer reviews and Q&As

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Question

We'd like to know whether endomorphin antibodies can be used for Western blotting. Product sheet states that they have not been tested for Westerns. Do the antibodies only recognise native proteins? Cat #s are ab10285, ab10289 and ab10290

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Answer

Thank you for your enquiry. Endomorphins are 4-amino-acid peptides with molecular weight ~ 480 Daltons. Peptides with such a small weight cannot be run by western blotting - there is no such a gel that would allow retain such small peptides. Secondly, endomorphin precursor protein (large size protein that can be run by western blotting) is not known at present. Therefore, western blot application is irrelevant for antibodies against endomorphins. However, these antibodies can be used to stain proteins after electrophoresis, but what they will recognize is not clear. I hope this information helps. Please do not hesitate to contact us if you need any more advice or information.

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Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"

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