Anti-Endomorphin 1 antibody (ab10285)

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Immunohistochemistry (Frozen sections) - Anti-Endomorphin 1 antibody (ab10285)

    Key features and details

    • Rabbit polyclonal to Endomorphin 1
    • Suitable for: IHC-Fr
    • Reacts with: Mouse, Rat, Human, Pig, Monkey
    • Isotype: IgG

    Overview

    • Product name

      Anti-Endomorphin 1 antibody

    • Description

      Rabbit polyclonal to Endomorphin 1

    • Host species

      Rabbit

    • Specificity

      Sensitivity has been determined by ELISA. Less than 1ng of antibody could be detected after binding to Endomorphin 1 which is conjugated to titer plate: Endomorphin 1 = 100 Endomorphin 2 = 5 Leu5-Enkephalin = <0.01 Met5-Enkephalin = <0.03 beta-Endorphin = <0.01

    • Tested applications

      Suitable for: IHC-Frmore details

    • Species reactivity

      Reacts with: Mouse, Rat, Human, Pig, Monkey

    • Immunogen

      Synthetic peptide conjugated to BSA.

    Properties

    • Form

      Liquid

    • Storage instructions

      Shipped at 4°C. Upon delivery aliquot and store at -20°C or -80°C. Avoid repeated freeze / thaw cycles.
    • Concentration information loading...

    • Purity

      Immunogen affinity purified

    • Purification notes

      Selectivity towards Endomorphin 1 has been achieved by separating fraction of affinity purified antibodies which did not bind to Endomorphin 2.

    • Clonality

      Polyclonal

    • Isotype

      IgG

    • Research areas

    Applications

    The Abpromise guarantee

    Our Abpromise guarantee covers the use of ab10285 in the following tested applications.

    The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

    Application Abreviews Notes
    IHC-Fr
    Use a concentration of 15 µg/ml.
    Notes
    IHC-Fr
    Use a concentration of 15 µg/ml.

    Target

    • Relevance

      The endogenous agonists for the mu-opiate receptors, endomorphin-1; Tyr-Pro-Trp-Phe-NH2, and endomorphin-2; Tyr-Pro-Phe-Phe-NH2 have been reported to show hypotensive activity and Ca2+ channel current inhibitory activity [Biochem. Biophys. Res. Commun.,235, 567 (1997)]. Although first identified in bovine brain, it is thought that endomorphin-1 and 2 are common mu-opiate agonists in mammals, including humans. Endomorphins may be useful for the study of pain perception together with some other biological functions in the body.

    • Alternative names

      • EM 1 antibody

    Images

    • Immunohistochemistry (Frozen sections) - Anti-Endomorphin 1 antibody (ab10285)
      Immunohistochemistry (Frozen sections) - Anti-Endomorphin 1 antibody (ab10285)
      ab10285 staining Endomorphin 1 in rat Peryaqueductal Grey Matter by Immunohistochemistry (PFA perfusion fixed frozen sections).

    References (1)

    Publishing research using ab10285? Please let us know so that we can cite the reference in this datasheet.

    ab10285 has been referenced in 1 publication.

    • Sun GC  et al. GPCR dimerization in brainstem nuclei contributes to the development of hypertension. Br J Pharmacol 172:2507-18 (2015). IHC ; Rat . PubMed: 25573074

    Customer reviews and Q&As

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    Question

    We'd like to know whether endomorphin antibodies can be used for Western blotting. Product sheet states that they have not been tested for Westerns. Do the antibodies only recognise native proteins? Cat #s are ab10285, ab10289 and ab10290

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    Answer

    Thank you for your enquiry. Endomorphins are 4-amino-acid peptides with molecular weight ~ 480 Daltons. Peptides with such a small weight cannot be run by western blotting - there is no such a gel that would allow retain such small peptides. Secondly, endomorphin precursor protein (large size protein that can be run by western blotting) is not known at present. Therefore, western blot application is irrelevant for antibodies against endomorphins. However, these antibodies can be used to stain proteins after electrophoresis, but what they will recognize is not clear. I hope this information helps. Please do not hesitate to contact us if you need any more advice or information.

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