Overview

  • Product name

  • Description

    Rabbit polyclonal to Endomucin
  • Host species

    Rabbit
  • Tested applications

    Suitable for: WBmore details
  • Species reactivity

    Reacts with: Human
  • Immunogen

    Recombinant fragment, corresponding to a region within amino acids 4-176 of Human Endomucin

  • Positive control

    • A431, HeLa and HepG2 cells

Properties

Applications

Our Abpromise guarantee covers the use of ab96315 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB 1/500 - 1/3000. Predicted molecular weight: 27 kDa.

Target

  • Function

    Endothelial sialomucin, also called endomucin or mucin-like sialoglycoprotein, which interferes with the assembly of focal adhesion complexes and inhibits interaction between cells and the extracellular matrix.
  • Tissue specificity

    Expressed in heart, kidney and lung.
  • Post-translational
    modifications

    Highly O-glycosylated. Sialic acid-rich glycoprotein.
  • Cellular localization

    Secreted and Cell membrane. Membrane. Consistently localized to the plasma membrane and less abundantly to Golgi-like perinuclear stacks.
  • Information by UniProt
  • Database links

  • Alternative names

    • EMCN antibody
    • EMCN2 antibody
    • Endomucin 2 antibody
    • Endomucin antibody
    • Endomucin-2 antibody
    • Endothelial sialomucin antibody
    • Gastric cancer antigen Ga34 antibody
    • MUC-14 antibody
    • MUC14 antibody
    • MUCEN_HUMAN antibody
    • Mucin 14 antibody
    • Mucin-14 antibody
    see all

Images

  • Anti-Endomucin antibody (ab96315) at 1/1000 dilution + A431 whole cell lysate at 30 µg

    Predicted band size: 27 kDa



    12% SDS PAGE

References

ab96315 has not yet been referenced specifically in any publications.

Customer reviews and Q&As

Application
Immunocytochemistry/ Immunofluorescence
Sample
Human Cell (Human Retinal Endothelial cells)
Permeabilization
No
Specification
Human Retinal Endothelial cells
Blocking step
BSA as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 3% · Temperature: 25°C
Fixative
Paraformaldehyde

Abcam user community

Verified customer

Submitted Aug 04 2017

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