Overview

  • Product name

    Anti-Endomucin antibody [V.7C7.1]
    See all Endomucin primary antibodies
  • Description

    Rat monoclonal [V.7C7.1] to Endomucin
  • Host species

    Rat
  • Tested applications

    Suitable for: WB, IP, IHC-P, IHC-Fr, Flow Cyt, IHC - Wholemountmore details
    Unsuitable for: FuncS
  • Species reactivity

    Reacts with: Mouse, Human
  • Immunogen

    Mouse Endomucin-IgG fusion protein

Properties

Applications

Our Abpromise guarantee covers the use of ab106100 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB 1/50. Predicted molecular weight: 27 kDa.
IP Use at an assay dependent concentration.
IHC-P Use at an assay dependent concentration.
IHC-Fr Use at an assay dependent concentration.
Flow Cyt 1/50.

ab18450 - Rat monoclonal IgG2a, is suitable for use as an isotype control with this antibody.

 

IHC - Wholemount Use at an assay dependent concentration.
  • Application notes
    Is unsuitable for FuncS.
  • Target

    • Function

      Endothelial sialomucin, also called endomucin or mucin-like sialoglycoprotein, which interferes with the assembly of focal adhesion complexes and inhibits interaction between cells and the extracellular matrix.
    • Tissue specificity

      Expressed in heart, kidney and lung.
    • Post-translational
      modifications

      Highly O-glycosylated. Sialic acid-rich glycoprotein.
    • Cellular localization

      Secreted and Cell membrane. Membrane. Consistently localized to the plasma membrane and less abundantly to Golgi-like perinuclear stacks.
    • Information by UniProt
    • Database links

    • Alternative names

      • EMCN antibody
      • EMCN2 antibody
      • Endomucin 2 antibody
      • Endomucin antibody
      • Endomucin-2 antibody
      • Endothelial sialomucin antibody
      • Gastric cancer antigen Ga34 antibody
      • MUC-14 antibody
      • MUC14 antibody
      • MUCEN_HUMAN antibody
      • Mucin 14 antibody
      • Mucin-14 antibody
      see all

    Images

    • ab106100 staining Endomucin in Mouse kidney tissue sections by Immunohistochemistry (IHC-P - paraformaldehyde-fixed, paraffin-embedded sections). Tissue was fixed with paraformaldehyde and blocked with 5% BSA / 5% Donkey Serum for an hour; antigen retrieval was by heat mediation in a Tris EDTA pH 9. Samples were incubated with primary antibody 1/100 for 14 hours at 4°C. An Alexa Fluor® 568 - conjugated Donkey anti-rat IgG polyclonal (1/400) was used as the secondary antibody.

      See Abreview

    • Immunohistochemical whole flat mounted mouse retinal tissue image. Primary antibody was used at a concentration of 1/100 and incubated at 4°C for 14 hours. A secondary donkey anti-rat Alexa Fluor® 488 preadsorbed antibody similar to ab150153 was used at a 1/400 concentration.

      See Abreview

    • Immuno fluorescence staining of bEND3 cells with ab106100 at a concentration of 1 μg/ml.

    References

    This product has been referenced in:

    • Ogunlade O  et al. Monitoring neovascularization and integration of decellularized human scaffolds using photoacoustic imaging. Photoacoustics 13:76-84 (2019). Read more (PubMed: 30805295) »
    • Zhu Y  et al. The association between CD31hiEmcnhi endothelial cells and bone mineral density in Chinese women. J Bone Miner Metab N/A:N/A (2019). Read more (PubMed: 30919130) »
    See all 18 Publications for this product

    Customer reviews and Q&As

    1-2 of 2 Abreviews or Q&A

    Application
    IHC - Wholemount
    Sample
    Mouse Tissue (Retina flat mount)
    Specification
    Retina flat mount

    Abcam user community

    Verified customer

    Submitted Aug 07 2015

    Application
    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
    Sample
    Mouse Tissue sections (Kidney)
    Antigen retrieval step
    Heat mediated - Buffer/Enzyme Used: Tris EDTA pH 9
    Permeabilization
    Yes - 0.5% Triton X-100
    Specification
    Kidney
    Blocking step
    5% BSA / 5% Donkey Serum as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 5%
    Fixative
    Paraformaldehyde

    Abcam user community

    Verified customer

    Submitted Aug 07 2015

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