Anti-Endomucin antibody [V.7C7.1] (ab106100)
Key features and details
- Rat monoclonal [V.7C7.1] to Endomucin
- Suitable for: IHC-Fr, IHC-P, IP, Flow Cyt, WB, IHC - Wholemount, ICC/IF
- Reacts with: Mouse, Human
- Isotype: IgG2a
Overview
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Product name
Anti-Endomucin antibody [V.7C7.1]
See all Endomucin primary antibodies -
Description
Rat monoclonal [V.7C7.1] to Endomucin -
Host species
Rat -
Tested applications
Suitable for: IHC-Fr, IHC-P, IP, Flow Cyt, WB, IHC - Wholemount, ICC/IFmore details
Unsuitable for: FuncS -
Species reactivity
Reacts with: Mouse, Human -
Immunogen
Mouse Endomucin-IgG fusion protein
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General notes
The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing this with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation. Please check that this product meets your needs before purchasing.
If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, along with publications, customer reviews and Q&As
Properties
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Form
Liquid -
Storage instructions
Shipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid freeze / thaw cycles. -
Storage buffer
Preservative: 0.02% Sodium azide
Constituents: 0.1% BSA, PBS -
Concentration information loading...
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Purity
Protein G purified -
Purification notes
0.2 µm filtered solution -
Clonality
Monoclonal -
Clone number
V.7C7.1 -
Isotype
IgG2a -
Research areas
Associated products
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Compatible Secondaries
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Isotype control
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Recombinant Protein
Applications
The Abpromise guarantee
Our Abpromise guarantee covers the use of ab106100 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
Application | Abreviews | Notes |
---|---|---|
IHC-Fr |
Use at an assay dependent concentration.
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IHC-P | (4) |
Use at an assay dependent concentration.
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IP |
Use at an assay dependent concentration.
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Flow Cyt |
Use at an assay dependent concentration.
ab18450 - Rat monoclonal IgG2a, is suitable for use as an isotype control with this antibody. |
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WB |
Use at an assay dependent concentration.
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|
IHC - Wholemount | (1) |
Use at an assay dependent concentration.
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ICC/IF | (1) |
Use a concentration of 1 µg/ml.
|
Notes |
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IHC-Fr
Use at an assay dependent concentration. |
IHC-P
Use at an assay dependent concentration. |
IP
Use at an assay dependent concentration. |
Flow Cyt
Use at an assay dependent concentration. ab18450 - Rat monoclonal IgG2a, is suitable for use as an isotype control with this antibody. |
WB
Use at an assay dependent concentration. |
IHC - Wholemount
Use at an assay dependent concentration. |
ICC/IF
Use a concentration of 1 µg/ml. |
Target
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Function
Endothelial sialomucin, also called endomucin or mucin-like sialoglycoprotein, which interferes with the assembly of focal adhesion complexes and inhibits interaction between cells and the extracellular matrix. -
Tissue specificity
Expressed in heart, kidney and lung. -
Post-translational
modificationsHighly O-glycosylated. Sialic acid-rich glycoprotein. -
Cellular localization
Secreted and Cell membrane. Membrane. Consistently localized to the plasma membrane and less abundantly to Golgi-like perinuclear stacks. - Information by UniProt
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Database links
- Entrez Gene: 51705 Human
- Entrez Gene: 59308 Mouse
- Omim: 608350 Human
- SwissProt: Q9ULC0 Human
- SwissProt: Q9R0H2 Mouse
- Unigene: 152913 Human
- Unigene: 27343 Mouse
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Alternative names
- EMCN antibody
- EMCN2 antibody
- Endomucin 2 antibody
see all
Protocols
To our knowledge, customised protocols are not required for this product. Please try the standard protocols listed below and let us know how you get on.
Datasheets and documents
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Datasheet download
References (41)
ab106100 has been referenced in 41 publications.
- Long JT et al. Hypertrophic chondrocytes serve as a reservoir for marrow-associated skeletal stem and progenitor cells, osteoblasts, and adipocytes during skeletal development. Elife 11:N/A (2022). PubMed: 35179487
- Maderna C et al. A murine model of cerebral cavernous malformations with acute hemorrhage. iScience 25:103943 (2022). PubMed: 35265815
- Crenn V et al. Signature of the vascular tumor microenvironment as a marker of the therapeutic response to doxorubicin in a preclinical model of osteosarcoma. Am J Cancer Res 12:1843-1854 (2022). PubMed: 35530297
- Palmisano B et al. GsαR201C and estrogen reveal different subsets of bone marrow adiponectin expressing osteogenic cells. Bone Res 10:50 (2022). PubMed: 35853852
- Lee SH et al. Single-cell transcriptomics reveal cellular diversity of aortic valve and the immunomodulation by PPARγ during hyperlipidemia. Nat Commun 13:5461 (2022). PubMed: 36115863