Recombinant
RabMAb

Recombinant Anti-Endostatin/COL18A1 antibody [EPR19995] (ab207162)

Overview

  • Product name

    Anti-Endostatin/COL18A1 antibody [EPR19995]
    See all Endostatin/COL18A1 primary antibodies
  • Description

    Rabbit monoclonal [EPR19995] to Endostatin/COL18A1
  • Host species

    Rabbit
  • Tested applications

    Suitable for: WBmore details
  • Species reactivity

    Reacts with: Human
  • Immunogen

    Recombinant fragment within Human Endostatin/COL18A1 aa 1550 to the C-terminus. The exact sequence is proprietary.
    Database link: P39060

  • Positive control

    • WB: Human placenta, seminoma, testis, fetal liver, fetal heart, fetal kidney and fetal spleen lysates; HepG2 whole cell lysate and supernatant.
  • General notes

     This product was previously labelled as Endostatin

     

    Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMab® patents.

    This product is a recombinant rabbit monoclonal antibody.

Properties

Applications

Our Abpromise guarantee covers the use of ab207162 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB 1/1000. Detects a band of approximately 20, 38, 50, 100, 120, 150, 180, 300 kDa (predicted molecular weight: 178 kDa).

Target

  • Function

    COLA18A probably plays a major role in determining the retinal structure as well as in the closure of the neural tube.
    Endostatin potently inhibits endothelial cell proliferation and angiogenesis. May inhibit angiogenesis by binding to the heparan sulfate proteoglycans involved in growth factor signaling.
  • Tissue specificity

    Present in multiple organs with highest levels in liver, lung and kidney.
  • Involvement in disease

    Defects in COL18A1 are a cause of Knobloch syndrome (KNO) [MIM:267750]. KNO is an autosomal recessive disorder defined by the occurrence of high myopia, vitreoretinal degeneration with retinal detachment, macular abnormalities and occipital encephalocele.
  • Sequence similarities

    Belongs to the multiplexin collagen family.
    Contains 1 FZ (frizzled) domain.
    Contains 1 TSP N-terminal (TSPN) domain.
  • Post-translational
    modifications

    Prolines at the third position of the tripeptide repeating unit (G-X-Y) are hydroxylated in some or all of the chains.
  • Cellular localization

    Secreted > extracellular space > extracellular matrix.
  • Information by UniProt
  • Database links

  • Alternative names

    • Alpha 1 collagen type 18 (XVIII)(COL18A1) antibody
    • Alpha 1 type XVIII collagen antibody
    • Antiangiogenic agent antibody
    • COIA1_HUMAN antibody
    • COL15A1 antibody
    • Col18a1 antibody
    • Collagen alpha 1(XV) chain antibody
    • Collagen alpha 1(XVIII) chain antibody
    • Collagen alpha-1(XV) chain antibody
    • Collagen type XV proteoglycan antibody
    • Collagen type XVIII alpha 1 antibody
    • Collagen XV, alpha 1 polypeptide antibody
    • Collagen, type XV, alpha 1 antibody
    • Endostatin antibody
    • Endostatin XV antibody
    • FLJ27325 antibody
    • FLJ34914 antibody
    • FLJ38566 antibody
    • KNO antibody
    • KNO1 antibody
    • KS antibody
    • MGC74745 antibody
    • Multi functional protein MFP antibody
    • OTTHUMP00000021782 antibody
    • OTTHUMP00000115472 antibody
    • OTTHUMP00000115473 antibody
    see all

Images

  • All lanes : Anti-Endostatin/COL18A1 antibody [EPR19995] (ab207162) at 1/1000 dilution

    Lane 1 : Human placenta lysate
    Lane 2 : U937 (Human histiocytic lymphoma cell line) whole cell lysate

    Lysates/proteins at 10 µg per lane.

    Secondary
    All lanes : Goat Anti-Rabbit IgG Peroxidase Conjugate, specific to the non-reduced form of IgG at 1/100000 dilution

    Predicted band size: 178 kDa


    Exposure time: 3 minutes


    Blocking/Dilution buffer: 5% NFDM/TBST.

    Negative control: U937 (PMID: 15451191).

  • All lanes : Anti-Endostatin/COL18A1 antibody [EPR19995] (ab207162) at 1/1000 dilution

    Lane 1 : Human seminoma lysate
    Lane 2 : Human testis lysate
    Lane 3 : Human fetal liver lysate
    Lane 4 : Human fetal heart lysate
    Lane 5 : Human fetal kidney lysate
    Lane 6 : Human fetal spleen lysate

    Lysates/proteins at 10 µg per lane.

    Secondary
    Lanes 1-3 : Goat Anti-Rabbit IgG Peroxidase Conjugate, specific to the non-reduced form of IgG at 1/100000 dilution
    Lanes 4-6 : Goat Anti-Rabbit IgG Peroxidase Conjugate, specific to the non-reduced form of IgG at 1/50000 dilution

    Predicted band size: 178 kDa



    Blocking/Dilution buffer: 5% NFDM/TBST.

    Exposure time: Lane 1/3: 1 minute; Lane 2/4-6: 3 minutes.

    The MW observed is consistent with the literature (PMID:10716919; PMID: 15451191; PMID: 9687493; PMID: 11581192; PMID: 15545970; PMID: 21555590).

  • All lanes : Anti-Endostatin/COL18A1 antibody [EPR19995] (ab207162) at 1/1000 dilution

    Lane 1 : HepG2 (Human liver hepatocellular carcinoma cell line) whole cell lysate
    Lane 2 : HepG2 (Human liver hepatocellular carcinoma cell line) supernatant

    Lysates/proteins at 10 µg per lane.

    Secondary
    Lane 1 : Goat Anti-Rabbit IgG H&L (HRP) (ab97051) at 1/50000 dilution
    Lane 2 : Goat Anti-Rabbit IgG H&L (HRP) (ab97051) at 1/100000 dilution

    Predicted band size: 178 kDa


    Exposure time: 3 minutes


    Blocking/Dilution buffer: 5% NFDM/TBST.

    Endostatin and NC1 fragments from proteolytic cleavage were secreted into culture supernatant as described by literature (PMID:1590618).

References

ab207162 has not yet been referenced specifically in any publications.

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