Anti-Endostatin/COL18A1 antibody [KT58] (ab114141)
Key features and details
- Mouse monoclonal [KT58] to Endostatin/COL18A1
- Suitable for: Sandwich ELISA
- Reacts with: Human
- Isotype: IgG1
Overview
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Product name
Anti-Endostatin/COL18A1 antibody [KT58]
See all Endostatin/COL18A1 primary antibodies -
Description
Mouse monoclonal [KT58] to Endostatin/COL18A1 -
Host species
Mouse -
Tested applications
Suitable for: Sandwich ELISAmore details -
Species reactivity
Reacts with: Human -
Immunogen
Recombinant full length protein corresponding to Human Endostatin/COL18A1.
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General notes
The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing this with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation. Please check that this product meets your needs before purchasing.
If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, along with publications, customer reviews and Q&As
Properties
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Form
Liquid -
Storage instructions
Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C or -80°C. Avoid freeze / thaw cycle. -
Storage buffer
Preservative: 0.1% Sodium azide
Constituent: 99% PBS -
Concentration information loading...
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Purity
Protein A purified -
Clonality
Monoclonal -
Clone number
KT58 -
Isotype
IgG1 -
Research areas
Associated products
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Compatible Secondaries
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Isotype control
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Recombinant Protein
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sELISA pair antibody
Applications
The Abpromise guarantee
Our Abpromise guarantee covers the use of ab114141 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
Application | Abreviews | Notes |
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Sandwich ELISA |
Use a concentration of 5 µg/ml. Can be paired for Sandwich ELISA with HRP Mouse monoclonal [KT60] to Endostatin/COL18A1 (ab106491).
For coating plates. |
Notes |
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Sandwich ELISA
Use a concentration of 5 µg/ml. Can be paired for Sandwich ELISA with HRP Mouse monoclonal [KT60] to Endostatin/COL18A1 (ab106491). For coating plates. |
Target
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Function
COLA18A probably plays a major role in determining the retinal structure as well as in the closure of the neural tube.
Endostatin potently inhibits endothelial cell proliferation and angiogenesis. May inhibit angiogenesis by binding to the heparan sulfate proteoglycans involved in growth factor signaling. -
Tissue specificity
Present in multiple organs with highest levels in liver, lung and kidney. -
Involvement in disease
Defects in COL18A1 are a cause of Knobloch syndrome (KNO) [MIM:267750]. KNO is an autosomal recessive disorder defined by the occurrence of high myopia, vitreoretinal degeneration with retinal detachment, macular abnormalities and occipital encephalocele. -
Sequence similarities
Belongs to the multiplexin collagen family.
Contains 1 FZ (frizzled) domain.
Contains 1 TSP N-terminal (TSPN) domain. -
Post-translational
modificationsProlines at the third position of the tripeptide repeating unit (G-X-Y) are hydroxylated in some or all of the chains. -
Cellular localization
Secreted > extracellular space > extracellular matrix. - Information by UniProt
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Database links
- Entrez Gene: 1306 Human
- Entrez Gene: 80781 Human
- Omim: 120328 Human
- SwissProt: P39059 Human
- SwissProt: P39060 Human
- Unigene: 409034 Human
- Unigene: 517356 Human
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Alternative names
- Alpha 1 collagen type 18 (XVIII)(COL18A1) antibody
- Alpha 1 type XVIII collagen antibody
- Antiangiogenic agent antibody
see all
Images
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ab114141, capturing Human Endostatin/COL18A1 in a Sandwich ELISA.
ab114141 was used at 5 µg/ml to coat the ELISA plates.
Detection utilised ab106491, which is an IgG2a monoclonal antibody conjugated to HRP.
Protocols
To our knowledge, customised protocols are not required for this product. Please try the standard protocols listed below and let us know how you get on.
Datasheets and documents
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SDS download
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Datasheet download
References (0)
ab114141 has not yet been referenced specifically in any publications.