Key features and details
- Mouse monoclonal [KT58] to Endostatin/COL18A1
- Suitable for: Sandwich ELISA
- Reacts with: Human
- Isotype: IgG1
Product nameAnti-Endostatin/COL18A1 antibody [KT58]
See all Endostatin/COL18A1 primary antibodies
DescriptionMouse monoclonal [KT58] to Endostatin/COL18A1
Tested applicationsSuitable for: Sandwich ELISAmore details
Species reactivityReacts with: Human
Recombinant full length protein corresponding to Human Endostatin/COL18A1.
This product was previously labelled as Endostatin
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In preparation for this, we have started to update the applications & species that this product is Abpromise guaranteed for.
We are also updating the applications & species that this product has been “predicted to work with,” however this information is not covered by our Abpromise guarantee.
Applications & species from publications and Abreviews that have not been tested in our own labs or in those of our suppliers are not covered by the Abpromise guarantee.
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Storage instructionsShipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C or -80°C. Avoid freeze / thaw cycle.
Storage bufferPreservative: 0.1% Sodium azide
Constituent: 99% PBS
Concentration information loading...
PurityProtein A purified
sELISA pair antibody
Our Abpromise guarantee covers the use of ab114141 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
|Sandwich ELISA||Use a concentration of 5 µg/ml. Can be paired for Sandwich ELISA with HRP Mouse monoclonal [KT60] to Endostatin/COL18A1 (ab106491). For coating plates.|
FunctionCOLA18A probably plays a major role in determining the retinal structure as well as in the closure of the neural tube.
Endostatin potently inhibits endothelial cell proliferation and angiogenesis. May inhibit angiogenesis by binding to the heparan sulfate proteoglycans involved in growth factor signaling.
Tissue specificityPresent in multiple organs with highest levels in liver, lung and kidney.
Involvement in diseaseDefects in COL18A1 are a cause of Knobloch syndrome (KNO) [MIM:267750]. KNO is an autosomal recessive disorder defined by the occurrence of high myopia, vitreoretinal degeneration with retinal detachment, macular abnormalities and occipital encephalocele.
Sequence similaritiesBelongs to the multiplexin collagen family.
Contains 1 FZ (frizzled) domain.
Contains 1 TSP N-terminal (TSPN) domain.
modificationsProlines at the third position of the tripeptide repeating unit (G-X-Y) are hydroxylated in some or all of the chains.
Cellular localizationSecreted > extracellular space > extracellular matrix.
- Information by UniProt
- Alpha 1 collagen type 18 (XVIII)(COL18A1) antibody
- Alpha 1 type XVIII collagen antibody
- Antiangiogenic agent antibody
ab114141, capturing Human Endostatin/COL18A1 in a Sandwich ELISA.
ab114141 was used at 5 µg/ml to coat the ELISA plates.
Detection utilised ab106491, which is an IgG2a monoclonal antibody conjugated to HRP.
To our knowledge, customised protocols are not required for this product. Please try the standard protocols listed below and let us know how you get on.
ab114141 has not yet been referenced specifically in any publications.