Overview

  • Product name
    Anti-Endothelial Cell antibody [1F10] (Biotin)
    See all Endothelial Cell primary antibodies
  • Description
    Rat monoclonal [1F10] to Endothelial Cell (Biotin)
  • Host species
    Rat
  • Conjugation
    Biotin
  • Specificity
    ab51675 recognises an antigen on human continuous blood endothelial cells, which are fully differentiated and does not stain any other cell in tissues or in peripheral blood. On sinusoidal endothelial cells of the liver or lymphatic organs the antigen is inconsistently expressed or absent. On isolated cells, the antigen is found in variable numbers on freshly isolated umbilical vein endothelial cells (HUVECs). Antigen expression on cultured HUVECs is dependent on the culture conditions. Best results were obtained (40-70% pos.) by daily renewal of FCS containing medium supplemented with endothelial cell conditioned supernatant whereas commercially available endothelial cell media apparently have no influence on the antigen expression.
  • Tested applications
    Suitable for: IHC-Frmore details
    Unsuitable for: IHC-P
  • Species reactivity
    Reacts with: Human
    Does not react with: Mouse
  • Immunogen

    Cultured HUVECs.

  • Positive control
    • Human placenta.

Properties

  • Form
    Liquid
  • Storage instructions
    Shipped at 4°C. Store at +4°C short term (1-2 weeks). Store at -20°C or -80°C. Avoid freeze / thaw cycle.
  • Storage buffer
    Preservative: 0.01% Thimerosal (merthiolate)
    Constituents: PBS, 10mg/ml BSA, pH 7.2
  • Concentration information loading...
  • Purity
    Protein A purified
  • Clonality
    Monoclonal
  • Clone number
    1F10
  • Isotype
    IgG2a
  • Research areas

Applications

Our Abpromise guarantee covers the use of ab51675 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
IHC-Fr Use a concentration of 1 µg/ml.
  • Application notes
    Is unsuitable for IHC-P.
  • Target

    • Relevance
      The endothelium is a single layer of cells lining various organs and cavities of the body, especially the blood vessels, heart, and lymphatic vessels.Endothelial cells line the entire circulatory system, from the heart to the smallest capillary. Endothelial cells are involved in many aspects of vascular biology, such as vasoconstriction and vasodilation, and hence the control of blood pressure; blood clotting (thrombosis & fibrinolysis); atherosclerosis; formation of new blood vessels (angiogenesis) and inflammation and swelling (oedema). Endothelial cells also control the passage of materials, and the transit of white blood cells, into and out of the bloodstream.

    References

    This product has been referenced in:
    • Goerdt S  et al. Characterization and differential expression of an endothelial cell-specific surface antigen in continuous and sinusoidal endothelial, in skin vascular lesions and in vitro. Exp Cell Biol 57:185-92 (1989). Read more (PubMed: 2599260) »
    See 1 Publication for this product

    Customer reviews and Q&As

    1-2 of 2 Abreviews or Q&A

    Answer

    Thank you for contacting us regarding your search for antibodies to endothelial phenotype.

    As we had spoken about, ab51675 is an excellent product against endothelial cell and is guaranteed to work in human samples in IHC-Fr. However as endothelial phenotype may be best be visualized if you are able to have specific targets which arise during differentiation or other important phase whether during emergence of the endothelial phenotype, before or after. We are happy to help customers find the most suitable products for their research purposes; however we are not specialized in this particular field and do not want to recommend a product that may be unsuitable. We encourage customers to consult the latest literature available through PubMed and other resources in order to find the most up-to-date information about their specific research interests.

    I am sorry that I could not be more helpful, but I hope that the available literature in this area can provide some clarification. Please do not hesitate to contact us again with other needs or with any questions about our products.



    Free Rabbit monoclonal antibody with any purchase of a primary antibody, while stocks last! Quote “RABMAB-XBSMG” in your next primary antibody order. For more information, visit the following link: https://www.abcam.com/index.html?pageconfig=resource&rid=15447

    Read More

    Answer

    Thank you for contacting us.

    Yes, ab51675 is biotin conjugated. Biotin is not fluorescent so it does not have an excitation and anemission wavelength. If you would like to detect the target by fluorescence, you could use ab51675 withafluorescent probe labelled streptavidin/avidin.


    I hope this information is helpful to you. Please do not hesitate to contact us if you need any more advice or information.

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    Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"

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