Overview

  • Product name
    Anti-Endothelin 1 antibody - C-terminal
    See all Endothelin 1 primary antibodies
  • Description
    Rabbit polyclonal to Endothelin 1 - C-terminal
  • Host species
    Rabbit
  • Tested applications
    Suitable for: IHC-P, WB, ICC/IFmore details
  • Species reactivity
    Reacts with: Human
  • Immunogen

    Synthetic peptide within Human Endothelin 1 aa 176-203 (C terminal) conjugated to keyhole limpet haemocyanin. The exact sequence is proprietary.
    Database link: P05305

  • Positive control
    • Human liver tissue; A549 cells and cell lysate, ZR 751, U251, MDA MB453 cell lysates.

Properties

Applications

Our Abpromise guarantee covers the use of ab170544 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
IHC-P 1/50 - 1/100. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.
WB 1/100 - 1/500. Predicted molecular weight: 24 kDa.
ICC/IF 1/10 - 1/50.

Target

  • Function
    Endothelins are endothelium-derived vasoconstrictor peptides.
  • Tissue specificity
    Expressed in lung, placental stem villi vessels and in cultured placental vascular smooth muscle cells.
  • Sequence similarities
    Belongs to the endothelin/sarafotoxin family.
  • Cellular localization
    Secreted.
  • Information by UniProt
  • Database links
  • Alternative names
    • ARCND3 antibody
    • Big endothelin-1 antibody
    • EDN1 antibody
    • EDN1_HUMAN antibody
    • ET-1 antibody
    • ET1 antibody
    • HDLCQ7 antibody
    • PPET1 antibody
    • Preproendothelin 1 antibody
    • Preproendothelin-1 antibody
    • QME antibody
    see all

Images

  • Immunohistochemistry analysis in formalin fixed, paraffin embedded Human liver tissue labeling Endothelin 1 with ab170544 at 1/50 dilution, followed by peroxidase conjugation of the secondary antibody and DAB staining.

  • Immunofluorescent analysis of  A549 cells labeling Endothelin 1 with ab170544 at 1/10 (green). DAPI was used to stain the cell nuclear (blue).

  • All lanes : Anti-Endothelin 1 antibody - C-terminal (ab170544) at 1/100 dilution

    Lane 1 : ZR 75 1 cell lysate
    Lane 2 : U251 cell lysate
    Lane 3 : MDA MB453 cell lysate
    Lane 4 : A549 cell lysate

    Lysates/proteins at 35 µg per lane.

    Developed using the ECL technique.

    Predicted band size: 24 kDa

References

ab170544 has not yet been referenced specifically in any publications.

Customer reviews and Q&As

There are currently no Customer reviews or Questions for ab170544.
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Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"

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